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Recombinant Mouse CTSB cell lysate

Cat.No. : CTSB-1885MCL
Product Overview : Mouse Cathepsin-B / CTSB derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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Source : Human cells
Species : Mouse
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : Ctsb cathepsin B [ Mus musculus ]
Official Symbol : CTSB
Synonyms : CTSB; cathepsin B; cathepsin B1; CB;
Gene ID : 13030
mRNA Refseq : NM_007798
Protein Refseq : NP_031824
Pathway : Antigen processing and presentation, organism-specific biosystem; Antigen processing and presentation, conserved biosystem; Lysosome, organism-specific biosystem; Lysosome, conserved biosystem; thyroid hormone biosynthesis, conserved biosystem;
Function : cysteine-type endopeptidase activity; cysteine-type peptidase activity; endopeptidase activity; hydrolase activity; kininogen binding; peptidase activity; peptide binding; protein complex binding; protein self-association;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (7)

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How does CTSB affect extracellular matrix remodeling in conditions like osteoarthritis? 01/28/2022

CTSB affects extracellular matrix remodeling, relevant in joint diseases like osteoarthritis.

What role does CTSB play in the pathology of neurodegenerative diseases, such as Alzheimer's? 03/29/2020

CTSB is implicated in neurodegenerative diseases, potentially contributing to protein aggregation and neuronal damage.

How do alterations in CTSB expression or activity affect tissue repair and fibrosis? 04/26/2019

Variations in CTSB expression impact tissue repair processes and the development of fibrosis in various organs.

What are the mechanisms by which CTSB modulates apoptosis and cell survival? 09/26/2018

CTSB regulates apoptosis and cell survival, influencing cell fate decisions under stress or damage.

How does CTSB influence the tumor microenvironment and cancer progression? 08/16/2018

CTSB can influence cancer progression by modulating the tumor microenvironment and facilitating tumor cell invasion.

How does CTSB (Cathepsin B) contribute to protein degradation and lysosomal function? 04/01/2018

CTSB is involved in lysosomal protein degradation, essential for cellular waste disposal and recycling.

What is the impact of CTSB in inflammatory responses and immune cell activation? 01/04/2018

CTSB plays a role in inflammation, impacting immune cell function and inflammatory responses.

Customer Reviews (3)

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Reviews
08/08/2021

    Exceptional protein engineering services, our trusted research partner.

    07/13/2021

      Reliable protein crystallography, key for our structural biology research.

      11/15/2018

        Swift protein quantification, improves data reliability in our work.

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