Recombinant Mouse CDH1 cell lysate
Cat.No. : | CDH1-1892MCL |
Product Overview : | Mouse CDH1 / E-cad / CD324 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls |
- Specification
- Gene Information
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Source : | Human cells |
Species : | Mouse |
Preparation method : | Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. |
Lysis buffer : | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Quality control Testing : | 12.5% SDS-PAGE Stained with Coomassie Blue |
Recommended Usage : | 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
Stability : | Samples are stable for up to twelve months from date of receipt at -80°C |
Storage Buffer : | 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Storage Instruction : | Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes. |
Gene Name : | Cdh1 cadherin 1 [ Mus musculus ] |
Official Symbol : | CDH1 |
Synonyms : | CDH1; cadherin 1; cadherin-1; ARC-1; E-cadherin; uvomorulin; epithelial cadherin; Um; UVO; Ecad; L-CAM; AA960649; MGC107495; |
Gene ID : | 12550 |
mRNA Refseq : | NM_009864 |
Protein Refseq : | NP_033994 |
Pathway : | Adaptive Immune System, organism-specific biosystem; Adherens junction, organism-specific biosystem; Adherens junction, conserved biosystem; Adherens junctions interactions, organism-specific biosystem; Apoptosis, organism-specific biosystem; Apoptotic cleavage of cell adhesionproteins, organism-specific biosystem; Apoptotic cleavage of cellular proteins, organism-specific biosystem; |
Function : | GTPase activating protein binding; RPTP-like protein binding; beta-catenin binding; calcium ion binding; gamma-catenin binding; protein binding; protein domain specific binding; protein phosphatase binding; |
Products Types
◆ Recombinant Protein | ||
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CDH1-0969H | Recombinant Human CDH1 Protein, GST-Tagged | +Inquiry |
CDH1-207H | Recombinant Human CDH1 Protein, His-tagged | +Inquiry |
CDH1-41H | Recombinant Human CDH1 Protein, C-8His tagged, Biotinylated | +Inquiry |
◆ Lysates | ||
CDH1-736RCL | Recombinant Rat CDH1 cell lysate | +Inquiry |
CDH1-938HCL | Recombinant Human CDH1 cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (6)
Ask a questionCDH1 gene mutations are usually passed on in an autosomal dominant manner, depending on the type and location of the mutation.
Carriers of CDH1 gene mutations should have gastroscopy, ultrasonography, and other related tests to monitor the development and progression of stomach cancer.
For people with mutations in the CDH1 gene, prophylactic total gastrectomy may reduce the risk of diffuse stomach cancer. There is currently no specific treatment to target CDH1 gene mutations.
CDH1 gene mutation is closely related to the occurrence of diffuse gastric cancer, which is a special subtype of gastric cancer with a high degree of invasion and malignancy.
The CDH1 gene encodes a protein called E-calcin that plays an important role in cell adhesion and tissue differentiation. Mutations in the CDH1 gene may affect these processes, resulting in abnormal cell adhesion and tissue differentiation.
CDH1 gene mutations may be associated with the development of a small number of breast cancers, because CDH1 also plays a role in regulating cell adhesion and tissue structure in breast tissue.
Customer Reviews (3)
Write a reviewThis recombinant protein has a very short half-life and shows a very high clearance rate, which makes its biological activity more stable.
The protein was highly purified and was not doped with other proteins or impurities.
CDH1 has similar activity to the native protein and is able to perform the expected biological functions.
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