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Recombinant Mouse CDH1 cell lysate

Cat.No. : CDH1-1892MCL
Product Overview : Mouse CDH1 / E-cad / CD324 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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  • Gene Information
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Source : Human cells
Species : Mouse
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : Cdh1 cadherin 1 [ Mus musculus ]
Official Symbol : CDH1
Synonyms : CDH1; cadherin 1; cadherin-1; ARC-1; E-cadherin; uvomorulin; epithelial cadherin; Um; UVO; Ecad; L-CAM; AA960649; MGC107495;
Gene ID : 12550
mRNA Refseq : NM_009864
Protein Refseq : NP_033994
Pathway : Adaptive Immune System, organism-specific biosystem; Adherens junction, organism-specific biosystem; Adherens junction, conserved biosystem; Adherens junctions interactions, organism-specific biosystem; Apoptosis, organism-specific biosystem; Apoptotic cleavage of cell adhesionproteins, organism-specific biosystem; Apoptotic cleavage of cellular proteins, organism-specific biosystem;
Function : GTPase activating protein binding; RPTP-like protein binding; beta-catenin binding; calcium ion binding; gamma-catenin binding; protein binding; protein domain specific binding; protein phosphatase binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (6)

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How about the inheritance pattern of CDH1 gene mutations? 09/25/2019

CDH1 gene mutations are usually passed on in an autosomal dominant manner, depending on the type and location of the mutation.

What screening is required for carriers of CDH1 gene mutations? 08/03/2019

Carriers of CDH1 gene mutations should have gastroscopy, ultrasonography, and other related tests to monitor the development and progression of stomach cancer.

Can CDH1 gene mutations be prevented or treated? 06/14/2019

For people with mutations in the CDH1 gene, prophylactic total gastrectomy may reduce the risk of diffuse stomach cancer. There is currently no specific treatment to target CDH1 gene mutations.

Which gastric cancer subtypes are associated with CDH1 mutations? 04/21/2019

CDH1 gene mutation is closely related to the occurrence of diffuse gastric cancer, which is a special subtype of gastric cancer with a high degree of invasion and malignancy.

What are the effects of CDH1 gene mutations on cell adhesion and tissue differentiation? 04/01/2019

The CDH1 gene encodes a protein called E-calcin that plays an important role in cell adhesion and tissue differentiation. Mutations in the CDH1 gene may affect these processes, resulting in abnormal cell adhesion and tissue differentiation.

What is the relationship between CDH1 gene mutations and breast cancer? 03/24/2019

CDH1 gene mutations may be associated with the development of a small number of breast cancers, because CDH1 also plays a role in regulating cell adhesion and tissue structure in breast tissue.

Customer Reviews (3)

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Reviews
04/22/2022

    This recombinant protein has a very short half-life and shows a very high clearance rate, which makes its biological activity more stable.

    05/27/2020

      The protein was highly purified and was not doped with other proteins or impurities.

      05/21/2019

        CDH1 has similar activity to the native protein and is able to perform the expected biological functions.

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