Recombinant Human FCGRT & B2M cell lysate
Cat.No. : | FCGRT & B2M-1925HCL |
Product Overview : | Human Human FCGRT & B2M Heterodimer derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls |
- Specification
- Gene Information
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Source : | Human cells |
Species : | Human |
Preparation method : | Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. |
Lysis buffer : | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Quality control Testing : | 12.5% SDS-PAGE Stained with Coomassie Blue |
Recommended Usage : | 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
Stability : | Samples are stable for up to twelve months from date of receipt at -80°C |
Storage Buffer : | 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Storage Instruction : | Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes. |
Gene Name : | FCGRT Fc fragment of IgG, receptor, transporter, alpha [ Homo sapiens ]; B2M beta-2-microglobulin [ Homo sapiens ] |
Official Symbol : | B2M |
Synonyms : | FCRN; alpha-chain; IgG receptor FcRn large subunit p51; FcRn alpha chain; neonatal Fc receptor; neonatal Fc-receptor for Ig; IgG Fc fragment receptor transporter alpha chain; immunoglobulin receptor, intestinal, heavy chain; major histocompatibility complex class I-like Fc receptor; beta-2-microglobulin; beta-2-microglobin; beta chain of MHC class I molecules |
Gene ID : | 2217; 567 |
mRNA Refseq : | NM_001136019; NM_004048 |
Protein Refseq : | NP_001129491; NP_004039 |
MIM : | 601437; 109700 |
UniProt ID : | P55899; P61769 |
Chromosome Location : | 19q13.3; 15q21-q22.2 |
Pathway : | Adaptive Immune System, organism-specific biosystem; Antigen processing and presentation, organism-specific biosystem; DAP12 signaling, organism-specific biosystem |
Function : | IgG binding; IgG receptor activity; beta-2-microglobulin binding; identical protein binding; protein binding |
Products Types
◆ Recombinant Protein | ||
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FCGRT-1500R | Recombinant Rhesus Macaque FCGRT Protein, His (Fc)-Avi-tagged | +Inquiry |
FCGRT-1210H | Recombinant Human FCGRT Protein, GST-tagged | +Inquiry |
FCGRT-3998H | Recombinant Human FCGRT Protein, GST-tagged | +Inquiry |
◆ Lysates | ||
FCGRT-6278HCL | Recombinant Human FCGRT 293 Cell Lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Ask a questionCustomer Reviews (3)
Write a reviewWith a long shelf life, a single bottle of the reagent can be used multiple times, which is especially valuable for budget-conscious laboratories.
It maintains its activity and effectiveness under various environmental conditions.
The reagent has a wide range of applications, catering to diverse needs in different laboratories, be it in biomedical research or the field of bioengineering.
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