Creative BioMart to Present at
                        BIO-Europe Spring Creative BioMart to Present at AACR Annual Meeting|Apr. 5-10, 2024|Booth #2953

Recombinant Human FCGRT & B2M cell lysate

Cat.No. : FCGRT & B2M-1925HCL
Product Overview : Human Human FCGRT & B2M Heterodimer derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
  • Specification
  • Gene Information
  • Related Products
Source : Human cells
Species : Human
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : FCGRT Fc fragment of IgG, receptor, transporter, alpha [ Homo sapiens ]; B2M beta-2-microglobulin [ Homo sapiens ]
Official Symbol : B2M
Synonyms : FCRN; alpha-chain; IgG receptor FcRn large subunit p51; FcRn alpha chain; neonatal Fc receptor; neonatal Fc-receptor for Ig; IgG Fc fragment receptor transporter alpha chain; immunoglobulin receptor, intestinal, heavy chain; major histocompatibility complex class I-like Fc receptor; beta-2-microglobulin; beta-2-microglobin; beta chain of MHC class I molecules
Gene ID : 2217; 567
mRNA Refseq : NM_001136019; NM_004048
Protein Refseq : NP_001129491; NP_004039
MIM : 601437; 109700
UniProt ID : P55899; P61769
Chromosome Location : 19q13.3; 15q21-q22.2
Pathway : Adaptive Immune System, organism-specific biosystem; Antigen processing and presentation, organism-specific biosystem; DAP12 signaling, organism-specific biosystem
Function : IgG binding; IgG receptor activity; beta-2-microglobulin binding; identical protein binding; protein binding

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Inquiry

  • Q&As
  • Reviews

Q&As (0)

Ask a question

Customer Reviews (3)

Write a review
Reviews
10/16/2019

    With a long shelf life, a single bottle of the reagent can be used multiple times, which is especially valuable for budget-conscious laboratories.

    07/24/2019

      It maintains its activity and effectiveness under various environmental conditions.

      04/11/2017

        The reagent has a wide range of applications, catering to diverse needs in different laboratories, be it in biomedical research or the field of bioengineering.

        Ask a Question for All FCGRT Products

        Required fields are marked with *

        My Review for All FCGRT Products

        Required fields are marked with *

        0

        Inquiry Basket

        cartIcon
        logo

        FOLLOW US

        Terms and Conditions        Privacy Policy

        Copyright © 2024 Creative BioMart. All Rights Reserved.

        Contact Us

        • /

        Stay Updated on the Latest Bioscience Trends