Recombinant Rat ERBB2 cell lysate
Cat.No. : | ERBB2-2010RCL |
Product Overview : | Rat ErbB2 / HER2 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls |
- Specification
- Gene Information
- Related Products
Source : | Human cells |
Species : | Rat |
Preparation method : | Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. |
Lysis buffer : | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Quality control Testing : | 12.5% SDS-PAGE Stained with Coomassie Blue |
Recommended Usage : | 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
Stability : | Samples are stable for up to twelve months from date of receipt at -80°C |
Storage Buffer : | 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Storage Instruction : | Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes. |
Gene Name : | Erbb2 v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) [ Rattus norvegicus ] |
Official Symbol : | ERBB2 |
Synonyms : | ERBB2; v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian); receptor tyrosine-protein kinase erbB-2; p185neu; C-erbB-2; p185erbB2; NEU proto-oncogene; proto-oncogene NEU; proto-oncogene c-ErbB-2; epidermal growth factor receptor-related protein; Avian erythroblastosis viral (v-erb-B2) oncogene homologue 2 (neuro/glioblastoma derived oncogene homolog); |
Gene ID : | 24337 |
mRNA Refseq : | NM_017003 |
Protein Refseq : | NP_058699 |
Pathway : | Adherens junction, organism-specific biosystem; Adherens junction, conserved biosystem; Alpha6-Beta4 Integrin Signaling Pathway, organism-specific biosystem; Axon guidance, organism-specific biosystem; Bladder cancer, organism-specific biosystem; Bladder cancer, conserved biosystem; Calcium signaling pathway, organism-specific biosystem; |
Function : | ATP binding; DNA binding; Hsp90 protein binding; RNA polymerase I core binding; RNA polymerase I core binding; glycoprotein binding; contributes_to growth factor binding; identical protein binding; nucleotide binding; protein C-terminus binding; protein binding; protein heterodimerization activity; protein heterodimerization activity; protein phosphatase binding; protein tyrosine kinase activity; protein tyrosine kinase activity; receptor activity; receptor signaling protein tyrosine kinase activity; transmembrane receptor protein tyrosine kinase activity; transmembrane receptor protein tyrosine kinase activity; transmembrane signaling receptor activity; ubiquitin protein ligase binding; |
Products Types
◆ Recombinant Protein | ||
ERBB2-14H | Active Recombinant Human ERBB2 Protein, Fc/His-tagged, Alexa Fluor® 647 conjugated | +Inquiry |
ERBB2-177H | Active Recombinant Human ERBB2 Protein, DDDDK-tagged | +Inquiry |
ERBB2-287H | Recombinant Human ERBB2 Protein | +Inquiry |
ERBB2-2118H | Recombinant Human ERBB2 Protein, MYC/DDK-tagged | +Inquiry |
ERBB2-1062H | Recombinant Human ERBB2 protein(Met1-Thr652), Biotinylated | +Inquiry |
◆ Lysates | ||
ERBB2-2658HCL | Recombinant Human ERBB2 cell lysate | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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