Recombinant Human CD9 cell lysate
Cat.No. : | CD9-2110HCL |
Product Overview : | Human CD9 / Tspan-29 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls |
- Specification
- Gene Information
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Source : | Human cells |
Species : | Human |
Preparation method : | Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. |
Lysis buffer : | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Quality control Testing : | 12.5% SDS-PAGE Stained with Coomassie Blue |
Recommended Usage : | 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
Stability : | Samples are stable for up to twelve months from date of receipt at -80°C |
Storage Buffer : | 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Storage Instruction : | Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes. |
Gene Name : | CD9 CD9 molecule [ Homo sapiens ] |
Official Symbol : | CD9 |
Synonyms : | CD9; CD9 molecule; CD9 antigen (p24) , MIC3; CD9 antigen; BA2; motility related protein 1; MRP 1; P24; TSPAN29; 5H9 antigen; tetraspanin-29; BA-2/p24 antigen; CD9 antigen (p24); leukocyte antigen MIC3; motility related protein-1; cell growth-inhibiting gene 2 protein; MIC3; MRP-1; BTCC-1; DRAP-27; TSPAN-29; FLJ99568; |
Gene ID : | 928 |
mRNA Refseq : | NM_001769 |
Protein Refseq : | NP_001760 |
MIM : | 143030 |
UniProt ID : | P21926 |
Chromosome Location : | 12p13 |
Pathway : | Hematopoietic cell lineage, organism-specific biosystem; Hematopoietic cell lineage, conserved biosystem; Hemostasis, organism-specific biosystem; Platelet activation, signaling and aggregation, organism-specific biosystem; Platelet degranulation, organism-specific biosystem; Response to elevated platelet cytosolic Ca2+, organism-specific biosystem; a6b1 and a6b4 Integrin signaling, organism-specific biosystem; |
Function : | integrin binding; protein binding; |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (5)
Ask a questionStudies have suggested that CD9 protein expression levels may be associated with chemoresistance in certain types of cancer, making it a potential predictive marker for chemotherapy response.
Studies have suggested that changes in CD9 protein expression in renal cells may be associated with the progression of chronic kidney disease, making it a potential biomarker for monitoring disease progression.
As of now, there are no FDA-approved diagnostic tests or therapies specifically targeting CD9 protein, but ongoing research may lead to future applications.
CD9 protein expression has been associated with cardiovascular diseases such as atherosclerosis and thrombosis, and its levels are being studied as a potential prognostic marker for these conditions.
CD9 protein is involved in the regulation of mast cell activation and degranulation, processes that are central to the development of asthma and allergic reactions.
Customer Reviews (3)
Write a reviewIts unique characteristics contribute to obtaining clear and detailed structural information, allowing researchers to gain insights into protein interactions and conformational changes.
The CD9 protein comes highly recommended for its exceptional performance in various research applications.
Its exceptional purity and stability make it an ideal choice for a wide range of applications, ensuring reliable and reproducible results.
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