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Recombinant Mouse EPHA4 cell lysate

Cat.No. : EPHA4-2136MCL
Product Overview : Mouse EPHA4 / HEK8 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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Source : Human cells
Species : Mouse
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : Epha4 Eph receptor A4 [ Mus musculus ]
Official Symbol : EPHA4
Synonyms : EPHA4; Eph receptor A4; ephrin type-A receptor 4; rabbit; snoRNA MBII-267; tyrosine-protein kinase receptor MPK-3; tyrosine-protein kinase receptor SEK-1; rb; Sek; Cek8; Hek8; Sek1; Tyro1; AI385584; 2900005C20Rik;
Gene ID : 13838
mRNA Refseq : NM_007936
Protein Refseq : NP_031962
Pathway : Axon guidance, organism-specific biosystem; Axon guidance, conserved biosystem;
Function : ATP binding; DH domain binding; GPI-linked ephrin receptor activity; PH domain binding; ephrin receptor activity; ephrin receptor binding; kinase activity; nucleotide binding; protein binding; protein kinase activity; protein tyrosine kinase activity; receptor activity; transferase activity; transferase activity, transferring phosphorus-containing groups; transmembrane receptor protein tyrosine kinase activity; transmembrane-ephrin receptor activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (7)

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How does EPHA4 contribute to physiological processes and what is its specific role? 09/21/2021

EPHA4 is a member of the ephrin receptor family of protein kinases that plays an important role in neural development and synaptic plasticity. It is involved in axon guidance, neuronal migration, and cell proliferation.

What roles does the protein EPHA4 play in neuronal development? 01/19/2020

EPHA4 plays a critical role in neuronal development as a member of the Eph receptor family, which are receptor tyrosine kinases involved in various cellular processes. Specifically, EPHA4 regulates axon growth and guidance, acting as a repulsive cue to guide axons in the developing nervous system. Its involvement extends to synaptic plasticity and neuronal survival. Dysregulation of EPHA4 has been linked to neurological disorders, including Alzheimer's disease and neuropathic pain. Further research on the precise mechanisms and downstream signaling pathways of EPHA4 will deepen our understanding of its role in neuronal development and potential therapeutic applications.

How is EPHA4 expression regulated during development? 07/20/2019

EPHA4 expression is tightly regulated during nervous system development, with its expression gradually increasing during embryonic development. Additionally, EPHA4 expression is spatially regulated, with high levels in specific regions of the nervous system. Regulation of EPHA4 expression is mediated by several mechanisms, including transcriptional and post-transcriptional mechanisms.

How is EPHA4 involved in synaptic plasticity? 04/03/2018

EPHA4 is essential for normal synaptic plasticity, with its activation leading to the modulation of various synaptic proteins, such as AMPA receptors, NMDA receptors, and EphB receptors. Dysregulation of EPHA4-mediated signaling has been implicated in multiple neurodegenerative disorders, affecting both synaptic plasticity and axon growth.

What are the signaling mechanisms activated by EPHA4? 03/31/2018

EPHA4 signaling is initiated by binding to its ligands, which leads to activation of downstream signaling pathways. Several signaling pathways are activated by EPHA4, including the MAP kinase pathway, the PI3K-Akt pathway, and the RhoA-ROCK pathway, which play critical roles in the regulation of actin cytoskeleton dynamics.

Can EPHA4 be targeted therapeutically to treat neurodegenerative diseases? 04/07/2017

EPHA4 has emerged as a promising therapeutic target for various neurodegenerative diseases, and several pharmacological inhibitors have been developed to target EPHA4 signaling. These inhibitors have shown efficacy in preclinical models of several neurodegenerative diseases, suggesting their potential in clinical applications.

What is the role of genetic variants in the EPHA4 gene in neurodegenerative diseases? 09/29/2016

There is growing evidence that genetic variants in the EPHA4 gene may contribute to the susceptibility to neurodegenerative diseases, such as Alzheimer's and Parkinson's disease. These variants have been shown to modify EPHA4 expression and function, and may contribute to disease pathogenesis via several mechanisms, including synaptic dysfunction and axon degeneration.

Customer Reviews (3)

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Reviews
03/11/2020

    The results interpretation process with this protein reagent is straightforward, making it suitable for beginners as well.

    10/25/2019

      The composition ratio of the reagent is highly accurate, ensuring the reliability of the experimental results.

      06/10/2016

        Astounding in both reliability and steadfastness, it confers irrefutable persuasion to the experiment.

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