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Recombinant Mouse ACHE cell lysate

Cat.No. : ACHE-2163MCL
Product Overview : Mouse Acetylcholinesterase / ACHE derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
  • Specification
  • Gene Information
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Source : Human cells
Species : Mouse
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : Ache acetylcholinesterase [ Mus musculus ]
Official Symbol : ACHE
Synonyms : ACHE; acetylcholinesterase; mE1a; mE1b; mE1c; mE1d; mE1e; mE1c-long;
Gene ID : 11423
mRNA Refseq : NM_009599
Protein Refseq : NP_033729
Pathway : Acetylcholine Synthesis, organism-specific biosystem; Biogenic Amine Synthesis, organism-specific biosystem; Cholinergic synapse, organism-specific biosystem; Glycerophospholipid metabolism, organism-specific biosystem; Glycerophospholipid metabolism, conserved biosystem; Neuronal System, organism-specific biosystem; Neurotransmitter Clearance In The Synaptic Cleft, organism-specific biosystem;
Function : acetylcholine binding; acetylcholinesterase activity; acetylcholinesterase activity; carboxylesterase activity; choline binding; cholinesterase activity; cholinesterase activity; collagen binding; hydrolase activity; laminin binding; laminin binding; protein homodimerization activity; protein self-association; serine hydrolase activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (8)

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When can I receive the product after ordering? 02/08/2023

The time required for different proteins varies, you can pay attention to the information on the product details page, or contact our experts directly.

Can ACHE-3333HFL products change tags? 12/02/2022

Sure. We can customize proteins according to customer needs, including different tags, expression system, protein length, etc.

Why the protein length of ACHE-101H product is 1-351aa? 11/19/2022

Because we can customize proteins of different lengths according to customer needs, please contact us if you are interested in this product.

What is the mechanism by which patients with Alzheimer's disease are treated with acetylcholinesterase inhibitors? 04/26/2022

Patients with Alzheimer disease often receive treatment with acetylcholinesterase inhibitors that mitigate symptoms by hindering ACh turnover.

In which tissues is acetylcholinesterase mainly distributed? 04/14/2021

Acetylcholinesterase is known to be distributed in nervous tissue such as the brainstem, cerebellum, and peripheral and autonomic nervous systems. Skeletal muscle also contains AChE with distribution patterns seemingly related to the type of muscle (fast versus slow twitch) and their specific function. AChE is also present on red blood cell membranes.

Does ACHE only work on terminating neurotransmission? 03/16/2021

Although the primary function of AChE is to terminate neural transmission, investigators have found that AChE also plays a role in neural development. During fetal development, AChE contributes to morphogenesis in addition to its main enzymatic function.

What form does acetylcholinesterase usually exist in? 07/29/2020

Acetylcholinesterase exists as a monomer that often polymerizes into a dimer with a disulfide bond. Along with Van der Waals forces, two dimers may be linked to become tetramers.

What is the role of ACHE? 02/04/2020

The primary role of AChE is to terminate neuronal transmission and signaling between synapses to prevent ACh dispersal and activation of nearby receptors. They are an important component of pesticides and nerve agents.

Customer Reviews (2)

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Reviews
12/12/2020

    I have already recommended creative biomart products to many colleagues.

    05/09/2019

      Received the product very quickly after placing the order, I am very satisfied.

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