Creative BioMart to Present at
                        BIO-Europe Spring Creative BioMart to Present at AACR Annual Meeting|Apr. 5-10, 2024|Booth #2953

Recombinant Human PLA2G1B cell lysate

Cat.No. : PLA2G1B-2200HCL
Product Overview : Human PLA2G1B / PLA2 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
  • Specification
  • Gene Information
  • Related Products
Source : Human cells
Species : Human
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : PLA2G1B phospholipase A2, group IB (pancreas) [ Homo sapiens ]
Official Symbol : PLA2G1B
Synonyms : PLA2G1B; phospholipase A2, group IB (pancreas); PLA2, PLA2A, PPLA2; phospholipase A2; group IB phospholipase A2; phosphatidylcholine 2-acylhydrolase 1B; PLA2; PLA2A; PPLA2; MGC119834; MGC119835;
Gene ID : 5319
mRNA Refseq : NM_000928
Protein Refseq : NP_000919
MIM : 172410
UniProt ID : P04054
Chromosome Location : 12q23-qter
Pathway : Arachidonic acid metabolism, organism-specific biosystem; Arachidonic acid metabolism, conserved biosystem; Ether lipid metabolism, organism-specific biosystem; Ether lipid metabolism, conserved biosystem; Fat digestion and absorption, organism-specific biosystem; Fat digestion and absorption, conserved biosystem; Fc epsilon RI signaling pathway, organism-specific biosystem;
Function : bile acid binding; calcium ion binding; calcium ion binding; calcium-dependent phospholipase A2 activity; cell surface binding; hydrolase activity; phospholipase A2 activity; phospholipase A2 activity; receptor binding; receptor binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Inquiry

  • Q&As
  • Reviews

Q&As (5)

Ask a question
How is PLA2G1B involved in cardiovascular diseases? 07/01/2020

PLA2G1B is associated with atherosclerosis and other cardiovascular conditions due to its involvement in lipid metabolism and inflammation.

Are there potential neuroprotective effects of modulating PLA2G1B activity? 12/19/2019

Modulating PLA2G1B activity could be explored for potential neuroprotective effects, given its involvement in processes related to neuroinflammation.

In what diseases is PLA2G1B dysregulation observed? 07/30/2018

Dysregulation of PLA2G1B has been linked to inflammatory disorders, cardiovascular diseases, and certain cancers.

Can PLA2G1B be a therapeutic target for inflammatory conditions? 11/10/2017

Yes, targeting PLA2G1B may be a potential therapeutic strategy for inflammatory diseases, as it plays a role in the production of inflammatory mediators.

Can PLA2G1B inhibitors be used for treating cancer? 09/18/2017

Inhibiting PLA2G1B may be explored as a therapeutic approach in cancer treatment by disrupting lipid metabolism and inhibiting cancer cell proliferation.

Customer Reviews (3)

Write a review
Reviews
03/12/2020

    Its exceptional purity and stability make it an ideal choice for a wide range of applications, ensuring reliable and reproducible results.

    10/16/2017

      The PLA2G1B protein comes highly recommended for its exceptional performance in various research applications.

      01/09/2016

        Its unique characteristics contribute to obtaining clear and detailed structural information, allowing researchers to gain insights into protein interactions and conformational changes.

        Ask a Question for All PLA2G1B Products

        Required fields are marked with *

        My Review for All PLA2G1B Products

        Required fields are marked with *

        0

        Inquiry Basket

        cartIcon
        logo

        FOLLOW US

        Terms and Conditions        Privacy Policy

        Copyright © 2024 Creative BioMart. All Rights Reserved.

        Contact Us

        • /

        Stay Updated on the Latest Bioscience Trends