Recombinant Human PLA2G1B cell lysate
Cat.No. : | PLA2G1B-2200HCL |
Product Overview : | Human PLA2G1B / PLA2 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls |
- Specification
- Gene Information
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Source : | Human cells |
Species : | Human |
Preparation method : | Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. |
Lysis buffer : | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Quality control Testing : | 12.5% SDS-PAGE Stained with Coomassie Blue |
Recommended Usage : | 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
Stability : | Samples are stable for up to twelve months from date of receipt at -80°C |
Storage Buffer : | 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Storage Instruction : | Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes. |
Gene Name : | PLA2G1B phospholipase A2, group IB (pancreas) [ Homo sapiens ] |
Official Symbol : | PLA2G1B |
Synonyms : | PLA2G1B; phospholipase A2, group IB (pancreas); PLA2, PLA2A, PPLA2; phospholipase A2; group IB phospholipase A2; phosphatidylcholine 2-acylhydrolase 1B; PLA2; PLA2A; PPLA2; MGC119834; MGC119835; |
Gene ID : | 5319 |
mRNA Refseq : | NM_000928 |
Protein Refseq : | NP_000919 |
MIM : | 172410 |
UniProt ID : | P04054 |
Chromosome Location : | 12q23-qter |
Pathway : | Arachidonic acid metabolism, organism-specific biosystem; Arachidonic acid metabolism, conserved biosystem; Ether lipid metabolism, organism-specific biosystem; Ether lipid metabolism, conserved biosystem; Fat digestion and absorption, organism-specific biosystem; Fat digestion and absorption, conserved biosystem; Fc epsilon RI signaling pathway, organism-specific biosystem; |
Function : | bile acid binding; calcium ion binding; calcium ion binding; calcium-dependent phospholipase A2 activity; cell surface binding; hydrolase activity; phospholipase A2 activity; phospholipase A2 activity; receptor binding; receptor binding; |
Products Types
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PLA2G1B-291H | Recombinant Human PLA2G1B Protein, MYC/DDK-tagged, C13 and N15-labeled | +Inquiry |
◆ Lysates | ||
PLA2G1B-2033MCL | Recombinant Mouse PLA2G1B cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (5)
Ask a questionPLA2G1B is associated with atherosclerosis and other cardiovascular conditions due to its involvement in lipid metabolism and inflammation.
Modulating PLA2G1B activity could be explored for potential neuroprotective effects, given its involvement in processes related to neuroinflammation.
Dysregulation of PLA2G1B has been linked to inflammatory disorders, cardiovascular diseases, and certain cancers.
Yes, targeting PLA2G1B may be a potential therapeutic strategy for inflammatory diseases, as it plays a role in the production of inflammatory mediators.
Inhibiting PLA2G1B may be explored as a therapeutic approach in cancer treatment by disrupting lipid metabolism and inhibiting cancer cell proliferation.
Customer Reviews (3)
Write a reviewIts exceptional purity and stability make it an ideal choice for a wide range of applications, ensuring reliable and reproducible results.
The PLA2G1B protein comes highly recommended for its exceptional performance in various research applications.
Its unique characteristics contribute to obtaining clear and detailed structural information, allowing researchers to gain insights into protein interactions and conformational changes.
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