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Recombinant Mouse CD34 cell lysate

Cat.No. : CD34-2232MCL
Product Overview : Mouse CD34 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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Source : Human cells
Species : Mouse
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : Cd34 CD34 antigen [ Mus musculus ]
Official Symbol : CD34
Synonyms : CD34; CD34 antigen; hematopoietic progenitor cell antigen CD34; cluster designation 34; AU040960;
Gene ID : 12490
mRNA Refseq : NM_001111059
Protein Refseq : NP_001104529
Pathway : Adaptive Immune System, organism-specific biosystem; Cell adhesion molecules (CAMs), organism-specific biosystem; Cell adhesion molecules (CAMs), conserved biosystem; Hematopoietic cell lineage, organism-specific biosystem; Hematopoietic cell lineage, conserved biosystem; Immune System, organism-specific biosystem; Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell, organism-specific biosystem;
Function : carbohydrate binding; sulfate binding; transcription factor binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (6)

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How is CD34 used in the assessment of bone marrow health? 12/21/2022

CD34 is employed as a marker to evaluate the status of bone marrow, aiding in the diagnosis and monitoring of disorders affecting the bone marrow.

How does CD34 impact the prognosis of hematologic diseases? 05/02/2022

CD34 expression levels are sometimes correlated with the prognosis of certain hematologic diseases, helping clinicians predict disease outcomes and plan appropriate interventions.

How does CD34 contribute to the understanding of hematologic malignancies? 04/15/2021

CD34 expression patterns are studied to gain insights into the pathogenesis and progression of hematologic malignancies, aiding in the development of targeted therapies.

Can CD34 be used to monitor the effectiveness of cancer treatments? 12/06/2020

Yes, changes in CD34 expression can be indicative of the response to cancer treatments, providing valuable information for patient management.

Are there any specific therapeutic applications of CD34? 11/01/2019

CD34 is a target for therapeutic interventions, and research is ongoing to explore its potential for developing treatments for various diseases.

Is CD34 exclusively used in hematological research, or does it have applications in other medical fields? 06/26/2018

While CD34 is prominently used in hematological research, its role in angiogenesis extends its applications to cardiovascular research and regenerative medicine.

Customer Reviews (4)

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Reviews
07/02/2022

    CD34 protein may have a specific binding affinity or interaction with its target molecules or receptors, making it a valuable tool for targeted therapies or diagnostic applications.

    04/06/2019

      In some cases, manufacturers may be open to collaborating with researchers to further investigate the applications and potential of CD34 protein.

      03/03/2018

        If researchers require modifications or specific variants of CD34 protein for their experiments, the manufacturer could potentially offer custom protein engineering services to meet their needs.

        01/30/2016

          This collaboration can involve sharing resources, expertise, and data to advance scientific understanding and therapeutic development.

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