Recombinant Human INSR cell lysate
Cat.No. : | INSR-2648HCL |
Product Overview : | Human Insulin Receptor / INSR / CD220 ( Short Isoform ) derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls |
- Specification
- Gene Information
- Related Products
Source : | Human cells |
Species : | Human |
Preparation method : | Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. |
Lysis buffer : | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Quality control Testing : | 12.5% SDS-PAGE Stained with Coomassie Blue |
Recommended Usage : | 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
Stability : | Samples are stable for up to twelve months from date of receipt at -80°C |
Storage Buffer : | 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Storage Instruction : | Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes. |
Gene Name : | INSR insulin receptor [ Homo sapiens ] |
Official Symbol : | INSR |
Synonyms : | INSR; insulin receptor; CD220; IR; HHF5; |
Gene ID : | 3643 |
mRNA Refseq : | NM_000208 |
Protein Refseq : | NP_000199 |
MIM : | 147670 |
UniProt ID : | P06213 |
Chromosome Location : | 19p13.3-p13.2 |
Pathway : | Adherens junction, organism-specific biosystem; Adherens junction, conserved biosystem; Aldosterone-regulated sodium reabsorption, organism-specific biosystem; Aldosterone-regulated sodium reabsorption, conserved biosystem; IRS activation, organism-specific biosystem; IRS-mediated signalling, organism-specific biosystem; IRS-related events, organism-specific biosystem; |
Function : | 3-phosphoinositide-dependent protein kinase binding; ATP binding; GTP binding; PTB domain binding; SH2 domain binding; insulin binding; insulin binding; insulin binding; insulin receptor substrate binding; insulin-activated receptor activity; insulin-like growth factor I binding; insulin-like growth factor II binding; insulin-like growth factor receptor binding; lipoic acid binding; nucleotide binding; phosphatidylinositol 3-kinase binding; protein binding; protein complex binding; protein domain specific binding; protein phosphatase binding; protein tyrosine kinase activity; protein tyrosine kinase activity; protein tyrosine kinase activity; receptor activity; receptor signaling protein tyrosine kinase activity; |
Products Types
◆ Recombinant Protein | ||
INSR-1260H | Recombinant Human INSR Protein, His-SUMO-tagged | +Inquiry |
INSR-5093H | Active Recombinant Human INSR Protein, GST-tagged | +Inquiry |
INSR-3163H | Recombinant Human INSR Protein, His (Fc)-Avi-tagged | +Inquiry |
INSR-5092H | Recombinant Human INSR Protein, GST-tagged | +Inquiry |
INSR-0997H | Recombinant Human INSR Protein (M1-S1382), eGFP, Strep II, 10×His tagged | +Inquiry |
◆ Lysates | ||
INSR-474HCL | Recombinant Human INSR cell lysate | +Inquiry |
◆ Assay kits | ||
Kit-1607 | INSR Internalization Assay Kit | +Inquiry |
Kit-1605 | U2OS INSR Bioassay Kit | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Inquiry
- Q&As
- Reviews
Q&As (7)
Ask a questionMalfunction or dysregulation of the insulin receptor (INSR) can lead to disturbances in metabolic health, contributing to conditions like diabetes.
INSR regulates glucose metabolism by facilitating the cellular uptake of glucose in response to insulin.
The insulin receptor (INSR) is prominently expressed in tissues such as the liver, muscle, and adipose tissue.
Genetic variations or mutations in the insulin receptor (INSR) are associated with certain metabolic disorders and insulin resistance.
In diabetes, the insulin receptor (INSR) contributes to the development of insulin resistance, impairing its normal signaling functions.
The insulin receptor (INSR) protein plays a crucial role in cellular processes, particularly in mediating the effects of insulin.
Upon insulin binding, the insulin receptor (INSR) activates downstream signaling pathways, including the PI3K-AKT pathway.
Customer Reviews (3)
Write a reviewThe compatibility with a range of lab equipment streamlined experimental procedures, saving both time and effort.
The product's support in the form of educational resources positively impacted the learning curve for our researchers.
The proactive engagement with customer insights contributed to the product's ongoing relevance in the scientific community.
Ask a Question for All INSR Products
Required fields are marked with *
My Review for All INSR Products
Required fields are marked with *
Inquiry Basket