Recombinant Mouse PDPN cell lysate
Cat.No. : | PDPN-2687MCL |
Product Overview : | Mouse Podoplanin derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls |
- Specification
- Gene Information
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Source : | Human cells |
Species : | Mouse |
Preparation method : | Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. |
Lysis buffer : | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Quality control Testing : | 12.5% SDS-PAGE Stained with Coomassie Blue |
Recommended Usage : | 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
Stability : | Samples are stable for up to twelve months from date of receipt at -80°C |
Storage Buffer : | 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Storage Instruction : | Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes. |
Gene Name : | Pdpn podoplanin [ Mus musculus ] |
Official Symbol : | PDPN |
Synonyms : | PDPN; podoplanin; aggrus; PA2.26 antigen; glycoprotein 38; transmembrane glycoprotein E11; T1a; Gp38; OTS-8; T1alpha; RANDAM-2; T1-alpha; |
Gene ID : | 14726 |
mRNA Refseq : | NM_010329 |
Protein Refseq : | NP_034459 |
Function : | NOT amino acid transmembrane transporter activity; NOT folic acid transporter activity; NOT water channel activity; NOT water transmembrane transporter activity; |
Products Types
◆ Recombinant Protein | ||
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PDPN-42H | Recombinant Human PDPN Protein, Fc/Avi-tagged | +Inquiry |
PDPN-4018R | Recombinant Rat PDPN Protein, His (Fc)-Avi-tagged | +Inquiry |
PDPN-133H | Recombinant Human PDPN Protein, His (Fc)-Avi-tagged | +Inquiry |
PDPN-3915H | Recombinant Human PDPN Protein (Ala23-Leu131), C-His tagged | +Inquiry |
◆ Lysates | ||
PDPN-470MCL | Recombinant Mouse PDPN cell lysate | +Inquiry |
PDPN-2517HCL | Recombinant Human PDPN cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (7)
Ask a questionPDPN facilitates cancer progression by promoting tumor cell migration and enhancing metastatic potential.
PDPN is crucial for lymphatic vessel development and maintenance, aiding in lymphatic system function.
Changes in PDPN expression contribute to fibrosis and lymphedema pathogenesis, affecting tissue structure and fluid balance.
PDPN is significant in forming and stabilizing blood-lymphatic vessel junctions, ensuring proper vascular function.
PDPN affects platelet aggregation and blood coagulation, playing a role in thrombosis and hemostasis.
PDPN expression on tumor cells alters their interaction with the immune system, impacting immune evasion.
PDPN plays a role in wound healing and tissue regeneration, influencing cell migration and tissue repair.
Customer Reviews (3)
Write a reviewSwift protein expression optimization support; greatly appreciated.
Accurate protein structural characterization; indispensable for our research.
Reliable mass spectrometry analysis; pivotal for proteomics experiments.
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