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Recombinant Human IL20RA cell lysate

Cat.No. : IL20RA-2720HCL
Product Overview : Human IL20Rα derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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  • Gene Information
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Source : Human cells
Species : Human
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : IL20RA interleukin 20 receptor, alpha [ Homo sapiens ]
Official Symbol : IL20RA
Synonyms : IL20RA; interleukin 20 receptor, alpha; interleukin-20 receptor subunit alpha; IL 20R1; ZCYTOR7; CRF2-8; IL-20RA; IL-20R-alpha; interleukin-20 receptor I; IL-20 receptor subunit alpha; class II cytokine receptor ZCYTOR7; cytokine receptor class-II member 8; cytokine receptor family 2 member 8; IL-20R1; FLJ40993;
Gene ID : 53832
mRNA Refseq : NM_014432
Protein Refseq : NP_055247
MIM : 605620
UniProt ID : Q9UHF4
Chromosome Location : 6q23.3
Pathway : Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Jak-STAT signaling pathway, organism-specific biosystem; Jak-STAT signaling pathway, conserved biosystem;
Function : receptor activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (10)

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What is the impact of IL20RA on cytokine secretion? 09/15/2021

IL20RA increases IL-17 and IL-22 production.

Does IL20RA expression correlate with disease severity in psoriasis? 10/07/2020

IL20RA expression correlates with disease severity in psoriasis.

What is IL20RA's role in autoimmune diseases? 05/06/2019

IL20RA is implicated in autoimmune diseases like psoriasis.

How does IL20RA modulate skin inflammation? 08/09/2018

IL20RA modulates skin inflammation by activating keratinocytes.

How does IL20RA expression change in cancerous tissues? 06/29/2018

IL20RA expression is upregulated in melanoma.

How does IL20RA influence the JAK-STAT signaling pathway? 12/26/2017

IL20RA activates the JAK-STAT signaling pathway.

Does IL20RA interact with other interleukin receptors? 09/17/2017

IL20RA interacts with IL-20 and IL-24 receptors.

How does IL20RA affect T-cell differentiation? 01/22/2017

IL20RA induces Th17 cell differentiation.

Does IL20RA have a role in cell migration? 08/09/2016

IL20RA promotes keratinocyte migration.

What post-translational modifications occur on IL20RA? 01/16/2016

IL20RA undergoes phosphorylation.

Customer Reviews (3)

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Reviews
07/12/2021

    Good for signaling studies.

    10/13/2017

      High specificity.

      10/03/2016

        Effective in skin models.

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