Recombinant Mouse CA12 cell lysate
Cat.No. : | CA12-3067MCL |
Product Overview : | Mouse Ca12 / Car12 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls |
- Specification
- Gene Information
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Source : | Human cells |
Species : | Mouse |
Preparation method : | Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. |
Lysis buffer : | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Quality control Testing : | 12.5% SDS-PAGE Stained with Coomassie Blue |
Recommended Usage : | 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
Stability : | Samples are stable for up to twelve months from date of receipt at -80°C |
Storage Buffer : | 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Storage Instruction : | Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes. |
Gene Name : | Car12 carbonic anyhydrase 12 [ Mus musculus ] |
Official Symbol : | Car12 |
Synonyms : | Ca12; AI314958; 2310047E01Rik; carbonic anhydrase 12; CA XII; CA-XII; carbonic anhydrase XII; carbonate dehydratase XII |
Gene ID : | 76459 |
mRNA Refseq : | NM_178396 |
Protein Refseq : | NP_848483 |
UniProt ID : | Q8CI85 |
Chromosome Location : | 9 C; 9 |
Pathway : | Metabolism, organism-specific biosystem; Nitrogen metabolism, organism-specific biosystem; Nitrogen metabolism, conserved biosystem |
Function : | carbonate dehydratase activity; lyase activity; metal ion binding |
Products Types
◆ Recombinant Protein | ||
CA12-423R | Recombinant Rhesus Macaque CA12 Protein, His (Fc)-Avi-tagged | +Inquiry |
CA12-1154M | Recombinant Mouse CA12 Protein, His (Fc)-Avi-tagged | +Inquiry |
CA12-260M | Active Recombinant Mouse CA12 protein(Met1-Ser301), His-tagged | +Inquiry |
Car12-1958M | Recombinant Mouse Car12 Protein, Myc/DDK-tagged | +Inquiry |
CA12-478H | Recombinant Human CA12 Protein, His (Fc)-Avi-tagged | +Inquiry |
◆ Lysates | ||
CA12-3061HCL | Recombinant Human CA12 cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (7)
Ask a questionKnown inhibitors can be identified through drug screening assays, and their impact on CA12 can be studied using kinetic studies and cell-based assays.
Cellular localization can be identified using immunofluorescence, subcellular fractionation, or electron microscopy.
CA12 has been implicated in tumor progression, particularly in hypoxic environments, by modulating pH and thus influencing tumor cell survival and metastasis. This can be assessed using cellular assays and tumor models.
Downstream pathways can be identified using pathway analysis tools, phosphoproteomics, and specific signal transduction assays.
The activity modulation under different pH conditions can be determined using enzymatic assays and pH shift experiments.
Expression changes in response to stressors can be analyzed using techniques like qPCR, Western blotting, or proteomics following cellular treatment with specific agents.
Genetic variants and their impact can be determined using structural models and functional assays comparing wild-type and mutant proteins.
Customer Reviews (3)
Write a reviewProfessional assistance, invaluable for complex protein experiments.
Cost-effective quantitation, ideal for budget-conscious labs.
Impressive expertise, accelerates our research progress.
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