Recombinant Human MAPK3

• Cat.No.: MAPK3-36H
• Product Overview: Recombinant full-length tag-free human ERK1 was expressed in E. coli cells.

Specification

• Description: The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described.
• Source: E.Coli
• Species: Human
• Form: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.
• Molecular Mass: ~44 kDa
• Purity: >90%
• Applications: Kinase Assay; Western Blot
• Storage: Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.2 ug/ul

Gene Information

• Gene Name: MAPK3 mitogen-activated protein kinase 3 [ Homo sapiens (human) ]
• Official Symbol: MAPK3
• Synonyms: MAPK3; mitogen-activated protein kinase 3; ERK1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; MGC20180; EC 2.7.11.24; Extracellular signal-regulated kinase 1; Insulin-stimulated MAP2 kinase; MAP kinase 1; Microtubule-associated protein 2 kinas; OTTHUMP00000174538; OTTHUMP00000174540; Microtubule-associated protein 2 kinase
• Gene ID: 5595
• mRNA Refseq: NM_002746
• Protein Refseq: NP_002737
• MIM: 601795
• UniProt ID: P27361
• Chromosome Location: 16p11.2
• Pathway: AGE/RAGE pathway; ALK1 signaling events; ATF-2 transcription factor network
• Function: ATP binding; MAP kinase activity; phosphatase binding