Creative BioMart to Present at
                        BIO-Europe Spring Creative BioMart to Present at AACR Annual Meeting|Apr. 5-10, 2024|Booth #2953

Human Brain Tissue Lysate

Cat.No. : Brain Tissue-7H
  • Specification
  • Gene Information
  • Related Products
Species : Human
Form : 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.
Concentration : 2.0 mg/ml
Tissue Type : Brain Tissue
Preparation method : Brain tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin). Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.
Safety Testing : This material has been tested by accepted techniques and has been found to be negative for HBsAg, HIV 1/2, and HCV. Additional patient information may be available upon request.
Diagnosis : Normal
Expiration Date : One year from date of receipt
Size : 0.1 mg, 0.5 mg
Storage Instruction : For short term storage of this tissue lysate, freeze in single use aliquots at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. For long term storage freeze working aliquots at -70°C. Under proper storage conditions the shelf life of this tissue lysate is 6 months from the date of receipt.
Applications : This lysate is for use in Western blotting, 10 μg to 20 μg per lane is recommended for mini gel.

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Inquiry

  • Q&As
  • Reviews

Q&As (0)

Ask a question

Customer Reviews (0)

Write a review

Ask a Question for All Brain Tissue Products

Required fields are marked with *

My Review for All Brain Tissue Products

Required fields are marked with *

0

Inquiry Basket

cartIcon
logo

FOLLOW US

Terms and Conditions        Privacy Policy

Copyright © 2024 Creative BioMart. All Rights Reserved.

Contact Us

  • /

Stay Updated on the Latest Bioscience Trends