Human Medulla Oblongata Tissue Lysate

Cat.No.: Medulla Oblongata-34H
Species: Human
Form: The human medulla oblongata tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors.
Concentration: 3 mg/ml
Tissue Type: Medulla Oblongata
Preparation method: Human medulla oblongata tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The human medulla oblongata tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the medulla oblongata tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The medulla oblongata tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.
Safety Testing: This material has been tested by accepted techniques and has been found to be negative for HBsAg, HIV 1/2, and HCV. Additional patient information may be available upon request.
Diagnosis: Normal
Expiration Date: One half year from date of receipt
Size: 0.1 mg, 0.5 mg
Storage Instruction: This lysate should be aliquoted in working amounts and may be stored for up to one month at 2-8°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. For longer term storage, freeze working aliquots at -70°C.
Applications: This lysate is for use in Western blotting, 10 μg to 20 μg per lane is recommended for mini gel.

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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