3T3 NIH Whole Cell Lysate

• Cat.No.: NIH 3T3-151M

Specification

• Species: Mouse
• Form: The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.
• Concentration: 2.0 mg/ml
• Cell line: NIH/3T3 (embryo)
• Tissue Type: Fibroblast from NIH Swiss mouse embryos
• Growth media: DMEM & 10% fetal bovine serum
• Preparation method: Mouse NIH/3T3 cell lysate was prepared by homogenization in modified RIPA buffer (50 mM Tris-HCl, pH 7.4, 1% Triton X-100, 0.2% sodium deoxycholate, 0.2% sodium dodecylsulfate (SDS), 1 mM sodium ethylenediaminetetraacetate, 1 mM phenylmethyl-sulfonyl fluoride, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin)). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% SDS, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.
• Expiration Date: One year from date of receipt
• Size: 0.1 mg, 0.5 mg
• Storage Instruction: This whole cell lysate is stable for three months at -20°C or at -70°C for up to one year.
• Applications: This lysate is for use in Western blotting, 10 μg to 20 μg per lane is recommended for mini gel.