"RAW 264.7" Related Products

RAW 264.7 Whole Cell Lysate

Cat.No.: RAW 264.7-153M
Species: Mouse
Form: The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.
Concentration: 2.0 mg/ml
Cell line: RAW 264.7 (Abelson murine leukemia virus-induced tumor)
Tissue Type: BALB/c Mouse (adult, Male) ascites, Abelson murine leukemia virus transformed; macrophage, Abelson murine leukemia virus-induced tumor
Growth media: DMEM & 10% fetal bovine serum
Preparation method: RAW 264.7 lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The RAW 264.7 lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.
Expiration Date: One year from date of receipt
Size: 0.1 mg, 0.5 mg
Storage Instruction: This whole cell lysate is stable for three months at -20°C or at -70°C for up to one year.
Applications: This lysate is for use in Western blotting, 10 μg to 20 μg per lane is recommended for mini gel.
Products Types

◆ Lysates

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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