Active Recombinant Human CTSD, His-tagged

• Cat.No.: CTSD-1649H
• Product Overview: Recombinant Human CTSD (Leu21-Leu412), fused with a C-terminal 10-His tag, was expressed in Mouse NSO Cells.

Specification

• Description: This gene encodes a lysosomal aspartyl protease composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. This proteinase, which is a member of the peptidase C1 family, has a specificity similar to but narrower than that of pepsin A. Transcription of this gene is initiated from several sites, including one which is a start site for an estrogen-regulated transcript. Mutations in this gene are involved in the pathogenesis of several diseases, including breast cancer and possibly Alzheimer disease.
• Source: Mammalian cells
• Species: Human
• Tag: His
• Predicted N Terminal: Leu21
• Form: Supplied as a 0.2 µm filtered solution in MES and NaCl.
• Bio-activity: Measured by its ability to cleave the fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH2.
• Molecular Mass: Predicted Molecular Mass: 44 kDaSDS-PAGE: 50 kDa, reducing conditions
• Endotoxin: < 1.0 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles.6 months from date of receipt, -20 to -70 °C as supplied.3 months, -20 to -70 °C under sterile conditions after opening.
• Reconstitution: Reconstitute at 100 µg/mL in sterile, deionized water.

Gene Information

• Gene Name: CTSD cathepsin D [ Homo sapiens (human) ]
• Official Symbol: CTSD
• Synonyms: CTSD; cathepsin D; CPSD; CLN10; MGC2311; cathepsin D; lysosomal aspartyl protease; lysosomal aspartyl peptidase; ceroid-lipofuscinosis, neuronal 10; Cathepsin D; EC 3.4.23.5; OTTHUMP00000042282; OTTHUMP00000196039; OTTHUMP00000198692
• Gene ID: 1509
• mRNA Refseq: NM_001909
• Protein Refseq: NP_001900
• MIM: 116840
• UniProt ID: P07339
• Chromosome Location: 11p15.5
• Pathway: Ceramide signaling pathway; Direct p53 effectors; LKB1 signaling events; Lysosome; Tuberculosis; thyroid hormone biosynthesis
• Function: aspartic-type endopeptidase activity; peptidase activity