|Source:||Human duodenum tissue|
|Preparation method:||Duodenum tumor tissue lysate was prepared by homogenization in lysis buffer (50 mM Tris-HCl pH7.4, 150 mM NaCl, 1 mM PMSF, 1 mM EDTA, 5 μg/ml aprotinin, 5 μg/ml leupeptin, 1% Triton x-100, 1% DOC, 0.1% SDS). Tissue and cell debris was removed by centrifugation. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.|
|Storage Buffer:||Duodenum Tumor lysate is supplied in SDS sample buffer containing 5% β-mercaptoethanol.|
|Storage Instruction:||Store at 2-8?C for continuous use. For extended storage, freeze working aliquots at -70?C. Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is half a year from the date of receipt.|
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