Recombinant Human TNFSF10 293 Cell Lysate
Cat.No. : | TNFSF10-892HCL |
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- Gene Information
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Description : | Antigen standard for tumor necrosis factor (ligand) superfamily, member 10 (TNFSF10) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection. |
Source : | HEK 293 cells |
Species : | Human |
Components : | This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol). |
Size : | 0.1 mg |
Storage Instruction : | Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment. |
Applications : | ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane. |
Gene Name : | TNFSF10 tumor necrosis factor (ligand) superfamily, member 10 [ Homo sapiens ] |
Official Symbol : | TNFSF10 |
Synonyms : | TNFSF10; tumor necrosis factor (ligand) superfamily, member 10; tumor necrosis factor ligand superfamily member 10; Apo 2L; CD253; TL2; TRAIL; Apo-2 ligand; TNF-related apoptosis inducing ligand TRAIL; tumor necrosis factor (ligand) family, member 10; chemokine tumor necrosis factor ligand superfamily member 10; tumor necrosis factor apoptosis-inducing ligand splice variant delta; APO2L; Apo-2L; |
Gene ID : | 8743 |
mRNA Refseq : | NM_001190942 |
Protein Refseq : | NP_001177871 |
MIM : | 603598 |
UniProt ID : | P50591 |
Chromosome Location : | 3q26 |
Pathway : | Activation of Pro-Caspase 8, organism-specific biosystem; Apoptosis, organism-specific biosystem; Apoptosis, organism-specific biosystem; Apoptosis, conserved biosystem; Apoptosis, organism-specific biosystem; Caspase-8 is formed from procaspase-8, organism-specific biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; |
Products Types
◆ Recombinant Protein | ||
TNFSF10-03H | Active Recombinant Human TNFSF10 Protein (114-281), Tag-Free | +Inquiry |
TNFSF10-851M | Recombinant Mouse TNFSF10 protein(Pro118-Asn291), His-tagged | +Inquiry |
Tnfsf10-6550M | Active Recombinant Mouse Tnfsf10 Protein | +Inquiry |
TNFSF10-454H | Recombinant Human TNFSF10 Protein, His-tagged | +Inquiry |
TNFSF10-253H | Recombinant Human TNFSF10 Protein | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (7)
Ask a questionTNFSF10 protein holds great therapeutic potential as a target for various diseases. Studies have shown that utilizing its apoptosis-inducing ability, novel treatment strategies can be developed for cancer. Furthermore, TNFSF10 protein can be utilized in the design of immunotherapies to enhance immune responses. However, further research is needed to optimize TNFSF10 therapeutic strategies and gain a deeper understanding of its specific mechanisms in disease development.
The cellular source of TNFSF10 protein is diverse, including tumor cells, lymphocytes, monocytes, and dendritic cells. Additionally, some epithelial cells and endothelial cells also express TNFSF10 protein.
TNFSF10 protein has diverse physiological functions in tissue remodeling. It promotes inflammation, angiogenesis, and fibrosis processes. Moreover, TNFSF10 protein is involved in immune regulation and tissue repair, playing a crucial role in maintaining normal tissue functionality and structure.
TNFSF10 modulates multiple cellular processes beyond apoptosis induction. It can activate various signaling pathways, including the NF-κB pathway, which plays a key role in immune and inflammatory responses. TNFSF10 also regulates cell migration and invasion properties, affecting metastasis in some cancer types. Furthermore, TNFSF10 can promote immune cell activation and cytokine production, contributing to the regulation of the immune system.
TNFSF10 protein plays a significant role in cancer progression and metastasis. It induces apoptosis in tumor cells, inhibits tumor growth and metastasis, and regulates immune responses. Moreover, TNFSF10 protein interacts with other signaling pathways, participating in the modulation of the tumor microenvironment.
The transcriptional regulation of TNFSF10 gene involves various factors. Known regulators include transcription factors NF-κB, STAT3, and DNA methylation status. These factors modulate the expression levels of TNFSF10 gene, thereby influencing the production of TNFSF10 protein.
TNFSF10 protein interacts with its receptor, death receptor 5 (DR5), and triggers apoptosis pathway to induce cell death. Additionally, TNFSF10 protein also interacts with other ligands and receptors, such as TRAIL-R1 and TRAIL-R2, thereby regulating cell survival and death signaling transduction.
Customer Reviews (3)
Write a reviewIts quality exceeding imagination, this experimental reagent becomes an indispensable tool for my experiments.
Through its employment, I am able to delve deeper into the essence of experimental principles and phenomena.
The exceptional performance of this experimental reagent significantly enhances the visibility of my research and amplifies my scholarly impact.
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