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Recombinant Human SOS1 293 Cell Lysate

Cat.No. : SOS1-1568HCL
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  • Gene Information
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Description : Antigen standard for son of sevenless homolog 1 (Drosophila) (SOS1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : SOS1 son of sevenless homolog 1 (Drosophila) [ Homo sapiens ]
Official Symbol : SOS1
Synonyms : SOS1; son of sevenless homolog 1 (Drosophila); GINGF, gingival fibromatosis, hereditary, 1; son of sevenless homolog 1; GF1; HGF; SOS-1; guanine nucleotide exchange factor; gingival fibromatosis, hereditary, 1; NS4; GGF1; GINGF;
Gene ID : 6654
mRNA Refseq : NM_005633
Protein Refseq : NP_005624
MIM : 182530
UniProt ID : Q07889
Chromosome Location : 2p21
Pathway : Activation of Rac, organism-specific biosystem; Acute myeloid leukemia, organism-specific biosystem; Acute myeloid leukemia, conserved biosystem; Adaptive Immune System, organism-specific biosystem; Antigen Activates B Cell Receptor Leading to Generation of Second Messengers, organism-specific biosystem; Axon guidance, organism-specific biosystem; B Cell Receptor Signaling Pathway, organism-specific biosystem;
Function : DNA binding; Ras guanyl-nucleotide exchange factor activity; Ras guanyl-nucleotide exchange factor activity; Rho GTPase activator activity; Rho guanyl-nucleotide exchange factor activity; protein binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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What is the volume of this reagent? 06/13/2023

The concentration of SOS1-13H is 1.0-2.0mg/ml. The volume can be calculated by the mass quantity and protein concentration.

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