Recombinant Human SMAD1 293 Cell Lysate

• Cat.No.: SMAD1-1678HCL

Specification

• Description: Antigen standard for SMAD family member 1 (SMAD1), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: SMAD1 SMAD family member 1 [ Homo sapiens ]
• Official Symbol: SMAD1
• Synonyms: SMAD1; SMAD family member 1; MAD, mothers against decapentaplegic homolog 1 (Drosophila) , MADH1, SMAD, mothers against DPP homolog 1 (Drosophila); mothers against decapentaplegic homolog 1; JV4 1; MADR1; MAD homolog 1; Mad-related protein 1; TGF-beta signaling protein 1; mothers against DPP homolog 1; SMAD, mothers against DPP homolog 1; MAD, mothers against decapentaplegic homolog 1; transforming growth factor-beta signaling protein 1; transforming growth factor-beta-signaling protein 1; BSP1; JV41; BSP-1; JV4-1; MADH1
• Gene ID: 4086
• mRNA Refseq: NM_005900
• Protein Refseq: NP_005891
• MIM: 601595
• UniProt ID: Q15797
• Chromosome Location: 4q31.21
• Pathway: ALK1 signaling events, organism-specific biosystem; ALK2 signaling events, organism-specific biosystem; BMP receptor signaling, organism-specific biosystem; Delta-Notch Signaling Pathway, organism-specific biosystem; ErbB1 downstream signaling, organism-specific biosystem; Heart Development, organism-specific biosystem; Id Signaling Pathway, organism-specific biosystem
• Function: I-SMAD binding; RNA polymerase II core promoter sequence-specific DNA binding; co-SMAD binding; identical protein binding; protein binding; protein kinase binding; receptor signaling protein activity; sequence-specific DNA binding transcription factor activity; transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity