Recombinant Human SCD 293 Cell Lysate

• Cat.No.: SCD-2044HCL

Specification

• Description: Antigen standard for stearoyl-CoA desaturase (delta-9-desaturase) (SCD) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: SCD stearoyl-CoA desaturase (delta-9-desaturase) [ Homo sapiens ]
• Official Symbol: SCD
• Synonyms: SCD; stearoyl-CoA desaturase (delta-9-desaturase); SCDOS, stearoyl CoA desaturase opposite strand; acyl-CoA desaturase; acyl CoA desaturase; delta 9 desaturase; FADS5; fatty acid desaturase; delta-9 desaturase; delta-9-desaturase; delta(9)-desaturase; predicted protein of HQ0998; stearoyl-CoA desaturase opposite strand; SCD1; SCDOS; MSTP008
• Gene ID: 6319
• mRNA Refseq: NM_005063
• Protein Refseq: NP_005054
• MIM: 604031
• UniProt ID: O00767
• Chromosome Location: 10q23-q24
• Pathway: Adipogenesis, organism-specific biosystem; Biosynthesis of unsaturated fatty acids, organism-specific biosystem; Biosynthesis of unsaturated fatty acids, conserved biosystem; Fatty Acid Biosynthesis, organism-specific biosystem; PPAR signaling pathway, organism-specific biosystem; PPAR signaling pathway, conserved biosystem; oleate biosynthesis II (animals), organism-specific biosystem
• Function: iron ion binding; oxidoreductase activity; oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water; stearoyl-CoA 9-desaturase activity