Recombinant Human RASGRP2 293 Cell Lysate

• Cat.No.: RASGRP2-2505HCL

Specification

• Description: Antigen standard for RAS guanyl releasing protein 2 (calcium and DAG-regulated) (RASGRP2), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: RASGRP2 RAS guanyl releasing protein 2 (calcium and DAG-regulated) [ Homo sapiens ]
• Official Symbol: RASGRP2
• Synonyms: RASGRP2; RAS guanyl releasing protein 2 (calcium and DAG-regulated); RAS guanyl-releasing protein 2; CALDAG GEFI; hCDC25L; cdc25-like protein; F25B3.3 kinase-like protein; guanine exchange factor MCG7; RAS guanyl nucleotide-releasing protein 2; calcium and DAG-regulated guanine nucleotide exchange factor I; calcium and diacylglycerol-regulated guanine nucleotide exchange factor I; CDC25L; CALDAG-GEFI
• Gene ID: 10235
• mRNA Refseq: NM_001098670
• Protein Refseq: NP_001092140
• MIM: 605577
• UniProt ID: Q7LDG7
• Chromosome Location: 11q13
• Pathway: Adaptive Immune System, organism-specific biosystem; Chemokine signaling pathway, organism-specific biosystem; Chemokine signaling pathway, conserved biosystem; Effects of PIP2 hydrolysis, organism-specific biosystem; G alpha (q) signalling events, organism-specific biosystem; GPCR downstream signaling, organism-specific biosystem; Hemostasis, organism-specific biosystem
• Function: calcium ion binding; calcium ion binding; diacylglycerol binding; guanyl-nucleotide exchange factor activity; lipid binding