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Recombinant Human PPM1B 293 Cell Lysate

Cat.No. : PPM1B-2963HCL
  • Specification
  • Gene Information
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Description : Antigen standard for protein phosphatase 1B (formerly 2C), magnesium-dependent, beta isoform (PPM1B), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : PPM1B protein phosphatase, Mg2+/Mn2+ dependent, 1B [ Homo sapiens ]
Official Symbol : PPM1B
Synonyms : PPM1B; protein phosphatase, Mg2+/Mn2+ dependent, 1B; protein phosphatase 1B (formerly 2C), magnesium dependent, beta isoform; protein phosphatase 1B; PP2CB; PP2CBETA; PPC2BETAX; protein phosphatase 2C; beta isoform; PP2C-beta; protein phosphatase 2C isoform beta; protein phosphatase 2C-like protein; protein phosphatase 1B (formerly 2C), magnesium-dependent, beta isoform; PP2C-beta-X; MGC21657;
Gene ID : 5495
mRNA Refseq : NM_002706
Protein Refseq : NP_002697
MIM : 603770
UniProt ID : O75688
Chromosome Location : 2p22.1
Pathway : Antiviral mechanism by IFN-stimulated genes, organism-specific biosystem; Cytokine Signaling in Immune system, organism-specific biosystem; ISG15 antiviral mechanism, organism-specific biosystem; Immune System, organism-specific biosystem; Interferon Signaling, organism-specific biosystem; MAPK signaling pathway, organism-specific biosystem; MAPK signaling pathway, organism-specific biosystem;
Function : hydrolase activity; magnesium ion binding; manganese ion binding; protein binding; protein serine/threonine phosphatase activity; protein serine/threonine phosphatase activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (7)

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How is PPM1B expression and activity controlled? 05/09/2022

Its expression and activity are regulated transcriptionally, post-transcriptionally, and post-translationally.

How does PPM1B regulate cellular stress responses? 03/12/2022

It mitigates stress by negatively regulating stress-activated protein kinases.

In which cellular pathways is PPM1B involved? 12/14/2020

PPM1B participates in p53 signaling, Wnt signaling, and the TGF-beta pathway.

What proteins does PPM1B interact with and regulate? 11/28/2020

It regulates multiple proteins, affecting their stability, localization, and function.

What are PPM1B’s primary biological functions? 05/16/2020

PPM1B dephosphorylates various substrates, influencing growth, development, and stress responses.

What is PPM1B's role in cell cycle regulation? 01/28/2020

PPM1B is involved in cell cycle progression.

What are the disease implications of PPM1B, and potential therapeutic approaches? 10/18/2019

PPM1B dysregulation is linked to diseases like cancer, and therapeutic strategies may include modulating its expression or activity.

Customer Reviews (3)

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Reviews
03/12/2022

    Perfect for my needs.

    12/14/2020

      Reliable and high quality.

      05/16/2020

        Good for protein experiments.

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