Recombinant Human NPFFR1 293 Cell Lysate
Cat.No. : | NPFFR1-3742HCL |
- Specification
- Gene Information
- Related Products
Description : | Antigen standard for neuropeptide FF receptor 1 (NPFFR1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection. |
Source : | HEK 293 cells |
Species : | Human |
Components : | This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol). |
Size : | 0.1 mg |
Storage Instruction : | Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment. |
Applications : | ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane. |
Gene Name : | NPFFR1 neuropeptide FF receptor 1 [ Homo sapiens ] |
Official Symbol : | NPFFR1 |
Synonyms : | NPFFR1; neuropeptide FF receptor 1; G protein coupled receptor 147 , GPR147; neuropeptide FF 1; NPFF1R1; OT7T022; G protein-coupled receptor 147; G-protein coupled receptor 147; RFamide-related peptide receptor OT7T022; NPFF1; GPR147; FLJ10751; |
Gene ID : | 64106 |
mRNA Refseq : | NM_022146 |
Protein Refseq : | NP_071429 |
MIM : | 607448 |
UniProt ID : | Q9GZQ6 |
Chromosome Location : | 10q21-q22 |
Pathway : | Class A/1 (Rhodopsin-like receptors), organism-specific biosystem; G alpha (q) signalling events, organism-specific biosystem; GPCR downstream signaling, organism-specific biosystem; GPCR ligand binding, organism-specific biosystem; GPCRs, Class A Rhodopsin-like, organism-specific biosystem; Neuroactive ligand-receptor interaction, organism-specific biosystem; Neuroactive ligand-receptor interaction, conserved biosystem; |
Function : | G-protein coupled receptor activity; neuropeptide receptor activity; receptor activity; signal transducer activity; |
Products Types
◆ Recombinant Protein | ||
NPFFR1-1842H | Recombinant Human NPFFR1 Protein, His&GST-tagged | +Inquiry |
NPFFR1-5933C | Recombinant Chicken NPFFR1 | +Inquiry |
◆ Assay kits | ||
Kit-1441 | cAMP NPFFR1 CHO-K1 GPCR Assay Kit | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Inquiry
0
Inquiry Basket