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Recombinant Human MTHFD2 293 Cell Lysate

Cat.No. : MTHFD2-4083HCL
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  • Gene Information
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Description : Antigen standard for methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase (MTHFD2), nuclear gene encoding mitochondrial protein, transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : MTHFD2 methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase [ Homo sapiens ]
Official Symbol : MTHFD2
Synonyms : MTHFD2; methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase; bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial; NAD-dependent methylene tetrahydrofolate dehydrogenase cyclohydrolase; NMDMC;
Gene ID : 10797
mRNA Refseq : NM_006636
Protein Refseq : NP_006627
MIM : 604887
UniProt ID : P13995
Chromosome Location : 2p13.1
Pathway : C1-unit interconversion, eukaryotes, organism-specific biosystem; C1-unit interconversion, eukaryotes, conserved biosystem; Metabolic pathways, organism-specific biosystem; Nucleotide Metabolism, organism-specific biosystem; One Carbon Metabolism, organism-specific biosystem; One carbon pool by folate, organism-specific biosystem; One carbon pool by folate, conserved biosystem;
Function : hydrolase activity; magnesium ion binding; methenyltetrahydrofolate cyclohydrolase activity; methylenetetrahydrofolate dehydrogenase (NAD+) activity; methylenetetrahydrofolate dehydrogenase (NADP+) activity; methylenetetrahydrofolate dehydrogenase (NADP+) activity; nucleotide binding; oxidoreductase activity; phosphate ion binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (5)

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How does MTHFD2 impact folate metabolism? 02/07/2021

MTHFD2 is involved in the mitochondrial folate pathway, influencing the synthesis of nucleotides and methionine.

Are there any ongoing clinical trials targeting MTHFD2? 10/07/2020

Some clinical trials are exploring the efficacy of MTHFD2 inhibitors in cancer treatment.

In what conditions is the expression of MTHFD2 altered? 06/29/2018

MTHFD2 expression is often dysregulated in cancer and other metabolic disorders.

Are there specific inhibitors or drugs targeting MTHFD2? 02/08/2018

Research is ongoing to develop inhibitors that selectively target MTHFD2, showing promise for cancer treatment.

Can MTHFD2 be used as a diagnostic marker for certain diseases? 09/17/2017

Elevated MTHFD2 levels have been observed in certain cancers, suggesting its potential as a diagnostic marker.

Customer Reviews (3)

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Reviews
11/15/2020

    It can also be employed in various molecular biology techniques, such as Western blotting, immunoprecipitation, or protein-protein interaction studies.

    02/01/2020

      Its versatility allows researchers to explore MTHFD2's involvement in diverse biological processes and fully uncover its functional role.

      02/17/2016

        the MTHFD2 protein's high quality, dependable performance, and comprehensive technical support make it an excellent choice for researchers seeking to investigate angiopoietin-related biology and unravel the complexities of vascular development and pathogenesis.

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