Recombinant Human PIK3CG, His-tagged

• Cat.No.: PIK3CG-53H
• Product Overview: Recombinant full length PI3Kg (126.3 kDa without Tag) produced inSf9carries an N-terminal His 8 affinity Tag.

Specification

• Cat. No.: PIK3CG-53H
• Description: PI3Kg is highly expressed in cells of hematopoietic origin. It plays an important role in dendritic cell (DC) trafficking and in the activation of specific immunity. PI3Kg-/- mice showed a reduced ability to respond to chemokines, and had a selective defect in the number of skin Langerhans cells and in lymph node CD8a-DCs. In macrophages, the chemokine RANTES/CCL5 activates the small GTPase Rac1 and its downstream target PAK2. This response depends on Gi activation and largely on the subsequent triggering of PI3Kg.
• Source: Sf9 insect cells.
• Physical Appearance: Sterile Filtered clear solution.
• Purity: Greater than 95.0% as determined by SDS-PAGE.
• Formulation: 0.5 mg/ml solution in 25mM HEPES, pH 8.0, 25mM NaCl, 2.5mM MgCl₂, and 50% glycerol.
• Biological Activity: 4.5 nmol/mg/min using phosphatidylinositol as the substrate.
• Storage: PI3Kg although stable at 14ºC for 1 week, should be stored desiccated below -18ºC. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Gene Information

• Gene Name: PIK3CG phosphoinositide-3-kinase, catalytic, gamma polypeptide [ Homo sapiens ]
• Synonyms: PIK3CG; phosphoinositide-3-kinase, catalytic, gamma polypeptide; PI3-kinase; p110-gamma; PTDINS-3-kinase; 1-phosphatidylinositol 3-kinase; phosphatidylinositol 3 kinase gamma, p110 gamma; phosphoinositide-3-kinase gamma catalytic subunit; hosphatidylinositol 3-kinase catalytic 110-kD gamma; phosphatidylinositol 3-kinase, catalytic, gamma polypeptide; EC 2.7.1.153; PI3-kinase p110 subunit gamma; PtdIns-3-kinase subunit p110; PI3Kgamma; PI3K; p120-PI3K
• Gene ID: 5294
• mRNA Refseq: NM_002649
• Protein Refseq: NP_002640
• MIM: 601232
• UniProt ID: P48736
• Chromosome Location: 7q22.3
• Pathway: Acute myeloid leukemia; Apoptosis; B cell receptor signaling pathway; Chemokine signaling pathway; Chronic myeloid leukemia; Colorectal cancer; Endometrial cancer; ErbB signaling pathway; Fc epsilon RI signaling pathway; Fc gamma R-mediated phagocytosis; Focal adhesion; Glioma; Inositol phosphate metabolism; Insulin signaling pathway; Jak-STAT signaling pathway; Leukocyte transendothelial migration; Melanoma; Natural killer cell mediated cytotoxicity; Neurotrophin signaling; Pancreatic cancer; Pathways in cancer; Phosphatidylinositol signaling system; Prostate cancer; Regulation of actin cytoskeleton; Renal cell carcinoma; Small cell lung cancer; T cell receptor signaling pathway; Toll-like receptor signaling pathway; Type II diabetes mellitus; VEGF signaling pathway; mTOR signaling pathway; Hemostasis; Signaling by GPCR
• Function: 1-phosphatidylinositol-3-kinase activity; ATP binding; inositol or phosphatidylinositol kinase activity; nucleotide binding; phosphatidylinositol-4,5-bisphosphate 3-kinase activity; phosphotransferase activity, alcohol group as acceptor; protein binding; transferase activity