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Recombinant Human ISL1 293 Cell Lysate

Cat.No. : ISL1-5149HCL
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  • Gene Information
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Description : Antigen standard for ISL LIM homeobox 1 (ISL1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : ISL1 ISL LIM homeobox 1 [ Homo sapiens ]
Official Symbol : ISL1
Synonyms : ISL1; ISL LIM homeobox 1; ISL1 transcription factor, LIM/homeodomain, (islet 1); insulin gene enhancer protein ISL-1; Isl 1; ISLET1; islet-1; ISL1 transcription factor, LIM/homeodomain; Isl-1;
Gene ID : 3670
mRNA Refseq : NM_002202
Protein Refseq : NP_002193
MIM : 600366
UniProt ID : P61371
Chromosome Location : 5q11.2
Pathway : Heart Development, organism-specific biosystem; Incretin Synthesis, Secretion, and Inactivation, organism-specific biosystem; Integration of energy metabolism, organism-specific biosystem; Metabolism, organism-specific biosystem; Regulation of Insulin Secretion, organism-specific biosystem; Synthesis, Secretion, and Inactivation of Glucose-dependent Insulinotropic Polypeptide (GIP), organism-specific biosystem;
Function : RNA polymerase II activating transcription factor binding; RNA polymerase II transcription coactivator activity; bHLH transcription factor binding; chromatin binding; enhancer sequence-specific DNA binding; enhancer sequence-specific DNA binding; estrogen receptor binding; ligand-dependent nuclear receptor binding; metal ion binding; sequence-specific DNA binding; sequence-specific DNA binding transcription factor activity; zinc ion binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (5)

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How is ISL1 protein utilized in clinical research? 03/16/2020

ISL1 protein is utilized in clinical research to better understand its role in development and disease, and to develop potential therapeutic interventions.

Are there any clinical implications of ISL1 protein in heart disease? 03/20/2019

Yes, ISL1 protein has been implicated in various heart diseases, including congenital heart defects and cardiac regeneration.

Are there any clinical trials targeting ISL1 protein for heart disease treatment? 12/16/2018

Some preclinical studies are exploring the potential of targeting ISL1 protein for cardiac regeneration and treatment of heart disease.

Are there any genetic disorders associated with ISL1 protein? 07/15/2018

Mutations in the ISL1 gene have been associated with certain congenital heart defects and developmental disorders.

Is there a link between ISL1 protein and neurodegenerative diseases? 02/21/2018

Some research suggests a potential link between ISL1 protein and neurodegenerative diseases, but further studies are needed to establish a definitive connection.

Customer Reviews (3)

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Reviews
12/31/2021

    Its stability and purity make it an ideal candidate for studying protein structures at a high resolution, providing crucial insights into the organization and function of the ISL1 protein.

    06/09/2020

      It has consistently demonstrated outstanding results, consistently producing clear and sharp bands, allowing for accurate and reliable protein detection and quantification.

      04/22/2019

        I highly recommend the ISL1 protein for its exceptional performance in Western blot analysis.

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