Recombinant Human IL19 293 Cell Lysate
Cat.No. : | IL19-5242HCL |
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- Gene Information
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Description : | Antigen standard for interleukin 19 (IL19), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection. |
Source : | HEK 293 cells |
Species : | Human |
Components : | This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol). |
Size : | 0.1 mg |
Storage Instruction : | Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment. |
Applications : | ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane. |
Gene Name : | IL19 interleukin 19 [ Homo sapiens ] |
Official Symbol : | IL19 |
Synonyms : | IL19; interleukin 19; interleukin-19; IL 10C; IL 19; MDA1; melanoma differentiation associated protein like protein; NG.1; ZMDA1; melanoma differentiation associated protein-like protein; melanoma differentiation-associated protein-like protein; IL-10C; |
Gene ID : | 29949 |
mRNA Refseq : | NM_013371 |
Protein Refseq : | NP_037503 |
MIM : | 605687 |
UniProt ID : | Q9UHD0 |
Chromosome Location : | 1q32.2 |
Pathway : | Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; IL23-mediated signaling events, organism-specific biosystem; Jak-STAT signaling pathway, organism-specific biosystem; Jak-STAT signaling pathway, conserved biosystem; |
Function : | cytokine activity; |
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Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (10)
Ask a questionConfocal microscopy and flow cytometry visualize IL19-induced cellular responses and track receptor internalization upon ligand binding.
Functional assays using immune cell cultures and cytokine profiling assays dissect IL19's impact on immune cell polarization and cytokine secretion.
Biophysical techniques like surface plasmon resonance and analytical ultracentrifugation quantify IL19's binding to IL20RA and IL20RB and reveal receptor complex composition.
Biophysical techniques such as surface plasmon resonance and isothermal titration calorimetry quantify IL19's binding kinetics to its receptor complexes.
CRISPR/Cas9 knockout models and siRNA-mediated knockdown in specific cell types unravel IL19's cell-specific roles.
Neutralizing antibodies, receptor blockade experiments, and in vivo disease models evaluate IL19's suitability as a therapeutic target in inflammatory and autoimmune diseases.
Recombinant protein expression in bacterial or mammalian systems followed by purification allows generation of functional IL19 for various experimental assays.
Reporter gene assays, ChIP, and DNA-protein interaction studies probe IL19's transcriptional regulation and interactions with specific DNA sequences.
Animal models and patient samples, combined with histopathology and molecular assays, shed light on IL19's involvement in skin disorders and its therapeutic potential.
Animal wound healing models, in vitro cell migration assays, and co-culture experiments investigate IL19's role in tissue repair and its effects on fibroblast and keratinocyte function.
Customer Reviews (3)
Write a reviewHigh reproducibility.
Suitable for Western blot.
Effective in cell differentiation.
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