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Recombinant Human IL19 293 Cell Lysate

Cat.No. : IL19-5242HCL
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  • Gene Information
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Description : Antigen standard for interleukin 19 (IL19), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : IL19 interleukin 19 [ Homo sapiens ]
Official Symbol : IL19
Synonyms : IL19; interleukin 19; interleukin-19; IL 10C; IL 19; MDA1; melanoma differentiation associated protein like protein; NG.1; ZMDA1; melanoma differentiation associated protein-like protein; melanoma differentiation-associated protein-like protein; IL-10C;
Gene ID : 29949
mRNA Refseq : NM_013371
Protein Refseq : NP_037503
MIM : 605687
UniProt ID : Q9UHD0
Chromosome Location : 1q32.2
Pathway : Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; IL23-mediated signaling events, organism-specific biosystem; Jak-STAT signaling pathway, organism-specific biosystem; Jak-STAT signaling pathway, conserved biosystem;
Function : cytokine activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (10)

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How are advanced imaging methods, like confocal microscopy and flow cytometry, applied to visualize IL19-mediated cellular responses and receptor internalization? 11/26/2022

Confocal microscopy and flow cytometry visualize IL19-induced cellular responses and track receptor internalization upon ligand binding.

What methods are employed to explore IL19's effects on immune cell polarization, cytokine secretion, and its potential involvement in chronic inflammatory conditions? 06/28/2022

Functional assays using immune cell cultures and cytokine profiling assays dissect IL19's impact on immune cell polarization and cytokine secretion.

What experimental strategies are utilized to investigate the binding affinities and stoichiometry of IL19 interactions with its cognate receptor IL20RA and potential co-receptor IL20RB? 03/30/2021

Biophysical techniques like surface plasmon resonance and analytical ultracentrifugation quantify IL19's binding to IL20RA and IL20RB and reveal receptor complex composition.

What techniques, such as surface plasmon resonance and isothermal titration calorimetry, are used to quantify IL19's binding kinetics to its receptor complexes? 08/14/2020

Biophysical techniques such as surface plasmon resonance and isothermal titration calorimetry quantify IL19's binding kinetics to its receptor complexes.

Could you elaborate on the use of CRISPR/Cas9-based knockout models and RNA interference to dissect IL19's role in specific cell types and tissues? 01/14/2020

CRISPR/Cas9 knockout models and siRNA-mediated knockdown in specific cell types unravel IL19's cell-specific roles.

What experimental methodologies, including neutralizing antibodies and receptor blockade, are employed to assess IL19's potential as a therapeutic target in inflammatory and autoimmune diseases? 12/18/2018

Neutralizing antibodies, receptor blockade experiments, and in vivo disease models evaluate IL19's suitability as a therapeutic target in inflammatory and autoimmune diseases.

How do researchers employ recombinant protein production techniques to generate bioactive IL19 for in vitro and in vivo functional studies? 11/08/2018

Recombinant protein expression in bacterial or mammalian systems followed by purification allows generation of functional IL19 for various experimental assays.

How are reporter gene assays, chromatin immunoprecipitation, and DNA-protein interaction techniques applied to uncover the transcriptional regulation of IL19 and its downstream targets? 11/03/2018

Reporter gene assays, ChIP, and DNA-protein interaction studies probe IL19's transcriptional regulation and interactions with specific DNA sequences.

How do researchers utilize animal models and patient samples to study IL19's contribution to skin disorders, such as psoriasis, and its therapeutic implications? 04/19/2017

Animal models and patient samples, combined with histopathology and molecular assays, shed light on IL19's involvement in skin disorders and its therapeutic potential.

Can you explain the experimental approaches to elucidate IL19's role in wound healing, tissue repair, and its impact on fibroblast and keratinocyte function? 02/18/2017

Animal wound healing models, in vitro cell migration assays, and co-culture experiments investigate IL19's role in tissue repair and its effects on fibroblast and keratinocyte function.

Customer Reviews (3)

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Reviews
02/22/2021

    High reproducibility.

    07/11/2020

      Suitable for Western blot.

      04/01/2019

        Effective in cell differentiation.

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