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Recombinant Human IL17B 293 Cell Lysate

Cat.No. : IL17B-5244HCL
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  • Gene Information
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Description : Antigen standard for interleukin 17B (IL17B) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : IL17B interleukin 17B [ Homo sapiens ]
Official Symbol : IL17B
Synonyms : IL17B; interleukin 17B; interleukin-17B; IL 17B; IL 20; MGC138900; MGC138901; neuronal interleukin 17 related factor; NIRF; ZCYTO7; interleukin 20; interleukin-20; cytokine Zcyto7; interleukin-17 beta; cytokine-like protein ZCYTO7; neuronal interleukin-17 related factor; neuronal interleukin-17-related factor; IL-20; IL-17B;
Gene ID : 27190
mRNA Refseq : NM_014443
Protein Refseq : NP_055258
MIM : 604627
UniProt ID : Q9UHF5
Chromosome Location : 5q33.1
Pathway : Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem;
Function : cytokine activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (10)

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What strategies are employed to investigate the cross-talk between IL17B and other cytokines in inflammatory responses? 09/01/2022

Co-culture experiments and signaling pathway analysis reveal IL17B's interaction with other cytokines.

How does IL17B's structure affect its receptor binding affinity, and what experimental techniques reveal this? 07/16/2022

Techniques like surface plasmon resonance elucidate IL17B's binding to its receptor.

How is the impact of IL17B on cancer progression studied using in vivo models? 05/01/2022

In vivo models combine IL17B overexpression or neutralization with tumor models to study its role.

What experimental designs are used to understand the role of IL17B in tissue-specific inflammation in human samples? 08/16/2021

Human tissue samples are analyzed using immunohistochemistry and cytokine profiling to understand IL17B's effects.

Can you elaborate on the techniques used to determine IL17B's role in modulating immune cell behavior? 12/16/2020

Flow cytometry and functional assays explore how IL17B affects immune cell activation and cytokine production.

What are the approaches to analyzing IL17B's involvement in neuroinflammation and central nervous system disorders? 07/05/2019

Research uses animal models and biomarker analysis to study IL17B in neuroinflammatory diseases.

Can you explain the use of genetic knockout or knockdown techniques to validate IL17B's functional effects? 05/24/2019

Genetic techniques like CRISPR/Cas9 knockout or siRNA knockdown validate IL17B's functional impact.

Could you detail the experimental protocols to assess the effect of IL17B on endothelial barrier function? 07/06/2018

Transwell assays and impedance-based methods evaluate IL17B's impact on endothelial barrier integrity.

How do researchers identify the downstream signaling pathways activated by IL17B and its receptor? 11/01/2017

Western blotting and phosphorylation assays uncover IL17B's downstream signaling pathways.

What experimental methods differentiate IL17B from other IL17 family members during gene expression analysis? 04/12/2017

Gene expression analysis involves qPCR or RNA sequencing to distinguish IL17B expression patterns.

Customer Reviews (3)

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Reviews
05/14/2022

    Suitable for signaling studies.

    10/30/2020

      High solubility

      10/10/2017

        Potent in B-cell assays.

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