Recombinant Human HSPA8 293 Cell Lysate

• Cat.No.: HSPA8-5353HCL

Specification

• Description: Antigen standard for heat shock 70kDa protein 8 (HSPA8), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: HSPA8 heat shock 70kDa protein 8 [ Homo sapiens ]
• Official Symbol: HSPA8
• Synonyms: HSPA8; heat shock 70kDa protein 8; heat shock 70kD protein 8 , HSPA10; heat shock cognate 71 kDa protein; HSC70; HSC71; HSP73; LPS-associated protein 1; heat shock 70kd protein 10; heat shock cognate protein 54; constitutive heat shock protein 70; lipopolysaccharide-associated protein 1; N-myristoyltransferase inhibitor protein 71; LAP1; HSC54; HSP71; NIP71; HSPA10; MGC29929; MGC131511
• Gene ID: 3312
• mRNA Refseq: NM_006597
• Protein Refseq: NP_006588
• MIM: 600816
• UniProt ID: P11142
• Chromosome Location: 11q24.1
• Pathway: Antigen processing and presentation, organism-specific biosystem; Antigen processing and presentation, conserved biosystem; C-MYB transcription factor network, organism-specific biosystem; Clathrin derived vesicle budding, organism-specific biosystem; Destabilization of mRNA by AUF1 (hnRNP D0), organism-specific biosystem; Diurnally regulated genes with circadian orthologs, organism-specific biosystem; Endocytosis, organism-specific biosystem
• Function: ATP binding; ATPase activity, coupled; nucleotide binding; protein binding