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Recombinant Human HPSE 293 Cell Lysate

Cat.No. : HPSE-5393HCL
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  • Gene Information
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Description : Antigen standard for heparanase (HPSE), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : HPSE heparanase [ Homo sapiens ]
Official Symbol : HPSE
Synonyms : HPSE; heparanase; HPA; HPSE1; HSE1; heparanase-1; endo-glucoronidase; HPA1; HPR1;
Gene ID : 10855
mRNA Refseq : NM_006665
Protein Refseq : NP_006656
MIM : 604724
UniProt ID : Q9Y251
Chromosome Location : 4q21.3
Pathway : Glycosaminoglycan degradation, organism-specific biosystem; Glycosaminoglycan degradation, conserved biosystem; Heparan sulfate degradation, organism-specific biosystem; Heparan sulfate degradation, conserved biosystem; Metabolic pathways, organism-specific biosystem; Syndecan-1-mediated signaling events, organism-specific biosystem;
Function : beta-glucuronidase activity; cation binding; hydrolase activity, acting on glycosyl bonds; protein binding; protein dimerization activity; syndecan binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (6)

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What is the role of HPSE protein in tumor immune evasion? 11/04/2022

By regulating the tumor microenvironment and the function of immune cells, HPSE protein participates in the process of tumor immune evasion.

Does HPSE protein have potential applications in tumor immunotherapy? 05/26/2022

Yes, the mechanism by which HPSE proteins act in relation to tumor immunotherapy has potential applications in this regard.

How about the fuction of HPSE protein in liver fibrosis? 09/30/2021

The protein plays an important role in liver fibrosis and participates in the regulation of liver tissue fibrosis through mechanisms such as collagen degradation.

How does HPSE protein participate in tumor invasion and metastasis? 08/25/2020

HPSE protein is involved in regulating tumor invasion and metastasis by degrading the basement membrane and promoting cytotoxicity.

What is the role of HPSE protein in collagen degradation, tissue repair and regeneration? 01/24/2020

The protein plays an important role in collagen degradation, tissue repair and regeneration, participates in epithelial-mesenchymal transformation processes, and affects biological processes such as cell migration and remodeling.

What is the role of HPSE protein in inflammatory response? 05/26/2019

HPSE proteins play an important role in the inflammatory response by participating in mechanisms such as extracellular matrix degradation and mediating cytokine production and release.

Customer Reviews (2)

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Reviews
12/08/2019

    The stability of HPSE was still very good under different temperature conditions, indicating that it has excellent thermal stability.

    05/19/2019

      Beacuse high efficiency and wide storage conditions of proteins make the experimental process more convenient and greatly improve the efficiency of scientific research.

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