Recombinant Human HPSE 293 Cell Lysate
Cat.No. : | HPSE-5393HCL |
- Specification
- Gene Information
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Description : | Antigen standard for heparanase (HPSE), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection. |
Source : | HEK 293 cells |
Species : | Human |
Components : | This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol). |
Size : | 0.1 mg |
Storage Instruction : | Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment. |
Applications : | ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane. |
Gene Name : | HPSE heparanase [ Homo sapiens ] |
Official Symbol : | HPSE |
Synonyms : | HPSE; heparanase; HPA; HPSE1; HSE1; heparanase-1; endo-glucoronidase; HPA1; HPR1; |
Gene ID : | 10855 |
mRNA Refseq : | NM_006665 |
Protein Refseq : | NP_006656 |
MIM : | 604724 |
UniProt ID : | Q9Y251 |
Chromosome Location : | 4q21.3 |
Pathway : | Glycosaminoglycan degradation, organism-specific biosystem; Glycosaminoglycan degradation, conserved biosystem; Heparan sulfate degradation, organism-specific biosystem; Heparan sulfate degradation, conserved biosystem; Metabolic pathways, organism-specific biosystem; Syndecan-1-mediated signaling events, organism-specific biosystem; |
Function : | beta-glucuronidase activity; cation binding; hydrolase activity, acting on glycosyl bonds; protein binding; protein dimerization activity; syndecan binding; |
Products Types
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Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (6)
Ask a questionBy regulating the tumor microenvironment and the function of immune cells, HPSE protein participates in the process of tumor immune evasion.
Yes, the mechanism by which HPSE proteins act in relation to tumor immunotherapy has potential applications in this regard.
The protein plays an important role in liver fibrosis and participates in the regulation of liver tissue fibrosis through mechanisms such as collagen degradation.
HPSE protein is involved in regulating tumor invasion and metastasis by degrading the basement membrane and promoting cytotoxicity.
The protein plays an important role in collagen degradation, tissue repair and regeneration, participates in epithelial-mesenchymal transformation processes, and affects biological processes such as cell migration and remodeling.
HPSE proteins play an important role in the inflammatory response by participating in mechanisms such as extracellular matrix degradation and mediating cytokine production and release.
Customer Reviews (2)
Write a reviewThe stability of HPSE was still very good under different temperature conditions, indicating that it has excellent thermal stability.
Beacuse high efficiency and wide storage conditions of proteins make the experimental process more convenient and greatly improve the efficiency of scientific research.
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