Recombinant Human HEY1 293 Cell Lysate

• Cat.No.: HEY1-5575HCL

Specification

• Description: Antigen standard for hairy/enhancer-of-split related with YRPW motif 1 (HEY1), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: HEY1 hairy/enhancer-of-split related with YRPW motif 1 [ Homo sapiens ]
• Official Symbol: HEY1
• Synonyms: HEY1; hairy/enhancer-of-split related with YRPW motif 1; hairy/enhancer-of-split related with YRPW motif protein 1; BHLHb31; CHF 2; CHF2; HERP2; HESR 1; HESR1; HRT 1; hHRT1; HES-related repressor protein 1; HES-related repressor protein 2; basic helix-loop-helix protein OAF1; hairy-related transcription factor 1; cardiovascular helix-loop-helix factor 2; class B basic helix-loop-helix protein 31; hairy and enhancer of split-related protein 1; OAF1; HRT-1; MGC1274
• Gene ID: 23462
• mRNA Refseq: NM_012258
• Protein Refseq: NP_036390
• MIM: 602953
• UniProt ID: Q9Y5J3
• Chromosome Location: 8q21
• Pathway: Delta-Notch Signaling Pathway, organism-specific biosystem; Heart Development, organism-specific biosystem; NOTCH1 Intracellular Domain Regulates Transcription, organism-specific biosystem; Notch-mediated HES/HEY network, organism-specific biosystem; Signal Transduction, organism-specific biosystem; Signaling by NOTCH, organism-specific biosystem; Signaling by NOTCH1, organism-specific biosystem
• Function: DNA binding; RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity; microsatellite binding; protein binding; protein binding transcription factor activity; sequence-specific DNA binding transcription factor activity; transcription factor binding