Recombinant Human GSN 293 Cell Lysate
Cat.No. : | GSN-5722HCL |
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- Gene Information
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Description : | Antigen standard for gelsolin (amyloidosis, Finnish type) (GSN), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection. |
Source : | HEK 293 cells |
Species : | Human |
Components : | This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol). |
Size : | 0.1 mg |
Storage Instruction : | Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment. |
Applications : | ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane. |
Gene Name : | GSN gelsolin [ Homo sapiens ] |
Official Symbol : | GSN |
Synonyms : | GSN; gelsolin; gelsolin (amyloidosis, Finnish type); amyloidosis; Finnish type; DKFZp313L0718; brevin; actin-depolymerizing factor; ADF; AGEL; |
Gene ID : | 2934 |
mRNA Refseq : | NM_000177 |
Protein Refseq : | NP_000168 |
MIM : | 137350 |
UniProt ID : | P06396 |
Chromosome Location : | 9q33 |
Pathway : | Amyloids, organism-specific biosystem; Apoptosis, organism-specific biosystem; Apoptotic cleavage of cellular proteins, organism-specific biosystem; Apoptotic executionphase, organism-specific biosystem; Caspase cascade in apoptosis, organism-specific biosystem; Caspase-mediated cleavage of cytoskeletal proteins, organism-specific biosystem; Coregulation of Androgen receptor activity, organism-specific biosystem; |
Function : | actin binding; calcium ion binding; protein binding; |
Products Types
◆ Recombinant Protein | ||
Gsn-3320M | Recombinant Mouse Gsn Protein, Myc/DDK-tagged | +Inquiry |
GSN-13H | Active Recombinant Human GSN Protein (125-150aa), N-6×His-tagged | +Inquiry |
GSN-2220H | Recombinant Human GSN Protein, His-tagged | +Inquiry |
GSN-1028H | Recombinant Human GSN Protein, His (Fc)-Avi-tagged | +Inquiry |
GSN-2221H | Recombinant Human GSN Protein, His-tagged | +Inquiry |
◆ Native Protein | ||
GSN-876P | Active Native Porcine GSN Protein | +Inquiry |
GSN-875B | Active Native Bovine GSN Protein | +Inquiry |
GSN-874P | Active Native Porcine GSN Protein | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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