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Recombinant Human GSDMD 293 Cell Lysate

Cat.No. : GSDMD-5725HCL
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  • Gene Information
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Description : Antigen standard for gasdermin D (GSDMD), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : GSDMD gasdermin D [ Homo sapiens ]
Official Symbol : GSDMD
Synonyms : GSDMD; gasdermin D; gasdermin domain containing 1 , GSDMDC1; gasdermin-D; DF5L; FLJ12150; gasdermin domain containing 1; gasdermin domain-containing protein 1; DFNA5L; FKSG10; GSDMDC1;
Gene ID : 79792
mRNA Refseq : NM_001166237
Protein Refseq : NP_001159709
UniProt ID : P57764
Chromosome Location : 8q24.3

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (5)

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Can GSDMD protein serve as a prognostic marker for patient outcomes in inflammatory diseases? 10/09/2021

Studies have suggested that the expression levels of GSDMD protein may be associated with disease severity and prognosis in certain inflammatory conditions, indicating its potential as a prognostic marker.

Can mutations in the GSDMD gene lead to clinical manifestations? 08/03/2021

Mutations in the GSDMD gene have been associated with autoinflammatory disorders, such as familial Mediterranean fever, which presents with recurrent episodes of fever and inflammation.

How is the activity of GSDMD protein regulated in the context of inflammatory diseases? 04/06/2018

Various signaling pathways and post-translational modifications regulate the activity of GSDMD protein, contributing to its involvement in the pathogenesis of inflammatory diseases.

How is the inflammasome linked to the function of GSDMD protein in clinical settings? 01/11/2017

The inflammasome, a multiprotein complex that regulates inflammatory responses, activates GSDMD protein to induce pyroptosis and release of pro-inflammatory cytokines, contributing to the pathogenesis of inflammatory diseases.

What are the potential diagnostic applications of targeting GSDMD protein? 04/24/2016

Targeting GSDMD protein could lead to the development of diagnostic tools for detecting and monitoring inflammatory diseases, providing valuable insights for patient management.

Customer Reviews (3)

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Reviews
06/18/2021

    Whether I need to study protein-protein interactions, protein localization, or protein folding, the GSDMD protein consistently delivers precise and meaningful data.

    07/27/2017

      the GSDMD protein's compatibility with various experimental techniques further supports its usability.

      01/19/2016

        One of the standout features of the GSDMD protein is the excellent technical support provided by the manufacturer.

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