Creative BioMart to Present at
                        BIO-Europe Spring Creative BioMart to Present at AACR Annual Meeting|Apr. 5-10, 2024|Booth #2953

Recombinant Human Signal Transducer And Activator Of Transcription 3

Cat.No. : STAT3-73H
Product Overview : Recombinant Human STAT3 encoding 50-240aa expressed inE.coliThe Stat3 protein is transcription factor expressed in most cell types and is activated by multiple cytokines and growth factors including: IFN-a, IL-10, IL-6, IL-11, IL-12, IL-2, EGF etc.
  • Specification
  • Gene Information
  • Related Products
Cat. No. : STAT3-73H
Description : STAT3 is tyrosine-phosphorylated and activated by a number of kinases. The binding of IL-6 family cytokines (including IL-6, oncostatin M and leukemia inhibitory factor) to the gp130 receptor triggers STAT3 phosphorylation by JAK2. EGF-R and certain other receptor tyrosine kinases, such as c-MET phosphorylate STAT3 in response to their ligands. STAT3 is also a target of the c-src non-receptor tyrosine kinase. STAT3-deficient mouse embryos can not develop beyond embryonic day 7 (E7.0), when gastrulation initiates. It appears that at these early stages of development, STAT3 activation is required for self-renewal of embryonic stem cells (ESCs). Indeed, LIF, which is supplied to ESC cultures to maintain their undifferentiated state, can be omitted if STAT3 is activated through some other means. Constitutive STAT3 activation is associated with various human cancers and commonly suggests poor prognosis. It has anti-apoptotic as well as proliferative effects.
Source : Escherichia Coli
Amino Acid Sequence : MGFWEEFESLQKQEVKNLHQRLEGQ RPENKGKNRYKNILPFDHSRVILQG RDSNIPGSDYINANYIKNQLLGPDE NAKTYIASQGCLEATVNDFWQMAWQ ENSRVIVMTTREVEKGRNKCVPYWP EVGMQRAYGPYSVTNCGEHDTTEYK LRTLQVSPLDNGDLIREIWHYQYLS WPDHGVPSEPGGVLSFLDQINQRQE SLPHAGPIIVHCSAGIGRTGTIIVI DMLMENISTKGLDCDIDIQKTIQMV RAQRSGMVQTEAQYKFIYVAIAQFI ETTKKKLEVLQSQKGQESEYGNITY
Physical Appearance : Sterile Filtered clear solution.
Purity : Greater than 95% as determined by SDS-PAGE.
Formulation : STAT3 at 50μg/ml in 50mM Tris-Acetate, pH7.5, 1mM EDTA, 20% Glycerol without BSA and Sodium Azide.
Application And Suggested Dilutions : • ELISA • Inhibition Assays • Western Blotting
Characterization : On SDS-PAGE commassie blue stained gel, the purified recombinant protein shows a band at 49 kDa.
Activity : Not known.
Storage : 1 week at 14ºC. For longer periods of time store vial at -20ºC to -80ºC.When stored at the recommended temperature, this protein is stable for 12 months. Avoid multiple freeze-thaw cycles.
Gene Name : STAT3 signal transducer and activator of transcription 3 (acute-phase response factor) [ Homo sapiens ]
Synonyms : STAT3; signal transducer and activator of transcription 3 (acute-phase response factor); APRF; HIES; FLJ20882; MGC16063; signal transducer and activator of transcription 3; DNA-binding protein APRF; acute-phase response factor
Gene ID : 6774
mRNA Refseq : NM_003150
Protein Refseq : NP_003141
MIM : 102582
UniProt ID : P40763
Chromosome Location : 17q21
Pathway : Acute myeloid leukemia; Adipocytokine signaling pathway; Chemokine signaling pathway; Jak-STAT signaling pathway; Pancreatic cancer; Pathways in cancer; Signaling by PDGF; Signalling by NGF
Function : calcium ion binding; hematopoietin/interferon-class (D200-domain) cytokine receptor signal transducer activity; protein dimerization activity; protein kinase binding; sequence-specific DNA binding; signal transducer activity; transcription activator activity; transcription factor activity; transcription factor binding

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Inquiry

  • Q&As
  • Reviews

Q&As (7)

Ask a question
Are there any known inhibitors targeting STAT3 for therapeutic purposes? 02/01/2022

Yes, there are several inhibitors targeting STAT3, including Stattic, S3I-201, and OPB-31121, which are being explored for their therapeutic potential in cancer and other diseases.

Can STAT3 activation influence cell proliferation and survival? 11/16/2021

Yes, activated STAT3 can promote cell proliferation and survival by inducing the expression of genes like cyclin D1 and Bcl-xL.

How does STAT3 contribute to oncogenesis and tumor progression? 04/12/2021

STAT3 can contribute to oncogenesis by promoting cell proliferation, survival, angiogenesis, and immune evasion, and by suppressing apoptosis in various cancer types.

How can the activation of STAT3 be detected in cells? 02/24/2021

Activation of STAT3 can be detected using Western blotting with phospho-specific antibodies that recognize the tyrosine-phosphorylated form of STAT3.

How does STAT3 interact with other signaling pathways in the cell? 08/25/2020

STAT3 can interact with other signaling pathways such as the MAPK, PI3K/AKT, and NF-κB pathways, leading to integrated cellular responses.

What are the primary upstream activators of STAT3? 01/02/2019

The primary upstream activators of STAT3 are cytokines and growth factors, especially those that signal through the Janus kinase (JAK) family, such as IL-6 and EGF.

What role does STAT3 play in immune cell differentiation and function? 04/16/2018

STAT3 plays a crucial role in the differentiation of Th17 cells, a subset of CD4+ T cells, and is also involved in the function of myeloid-derived suppressor cells (MDSCs) and regulatory T cells, influencing immune responses.

Customer Reviews (3)

Write a review
Reviews
11/17/2022

    Protein worked well in assay.

    04/21/2021

      Fast shipping, great customer service.

      02/06/2018

        Excellent purity, highly recommend.

        Ask a Question for All STAT3 Products

        Required fields are marked with *

        My Review for All STAT3 Products

        Required fields are marked with *

        0

        Inquiry Basket

        cartIcon
        logo

        FOLLOW US

        Terms and Conditions        Privacy Policy

        Copyright © 2024 Creative BioMart. All Rights Reserved.

        Contact Us

        • /

        Stay Updated on the Latest Bioscience Trends