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Recombinant Human GLB1 293 Cell Lysate

Cat.No. : GLB1-5909HCL
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  • Gene Information
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Description : Antigen standard for galactosidase, beta 1 (GLB1), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : GLB1 galactosidase, beta 1 [ Homo sapiens ]
Official Symbol : GLB1
Synonyms : GLB1; galactosidase, beta 1; elastin receptor 1 (67kD) , elastin receptor 1, 67kDa , ELNR1; beta-galactosidase; EBP; lactase; acid beta-galactosidase; elastin receptor 1, 67kDa; ELNR1; MPS4B;
Gene ID : 2720
mRNA Refseq : NM_000404
Protein Refseq : NP_000395
MIM : 611458
UniProt ID : P16278
Chromosome Location : 3p22.3
Pathway : Galactose metabolism, organism-specific biosystem; Galactose metabolism, conserved biosystem; Glycosaminoglycan degradation, organism-specific biosystem; Glycosaminoglycan degradation, conserved biosystem; Glycosphingolipid biosynthesis - ganglio series, organism-specific biosystem; Glycosphingolipid biosynthesis - ganglio series, conserved biosystem; Glycosphingolipid metabolism, organism-specific biosystem;
Function : beta-galactosidase activity; cation binding; galactoside binding; hydrolase activity, hydrolyzing O-glycosyl compounds; protein binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (5)

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How does GLB1 protein deficiency impact the nervous system? 07/04/2022

GLB1 deficiency can lead to neurodegeneration, causing progressive deterioration of the nervous system.

What are the different subtypes of GLB1 deficiency? 02/15/2020

There are three subtypes: GM1 gangliosidosis, mucopolysaccharidosis IVB, and combined GM1 gangliosidosis and mucopolysaccharidosis IVB.

Can GLB1 deficiency be managed through dietary interventions? 12/11/2019

While dietary interventions may help manage certain symptoms, they do not address the underlying cause of the deficiency.

What are the symptoms of GLB1 deficiency? 08/29/2018

Symptoms may include developmental delay, skeletal abnormalities, vision and hearing loss, and neurological deterioration.

Can GLB1 deficiency be detected prenatally? 05/21/2016

Yes, prenatal testing through genetic analysis of amniotic fluid or chorionic villus sampling can detect GLB1 deficiency.

Customer Reviews (3)

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12/10/2021

    Their support has been invaluable in troubleshooting and providing efficient solutions, enhancing the overall efficiency and productivity of my experiments.

    04/03/2019

      Its versatility allows for exploring its various roles and functions, shedding light on its significance in crucial biochemical pathways and cellular processes.

      05/16/2016

        Their knowledgeable and responsive team has consistently demonstrated their ability to address any concerns or challenges I have encountered along the way.

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