Recombinant Human EGR2 293 Cell Lysate

• Cat.No.: EGR2-6691HCL

Specification

• Description: Antigen standard for early growth response 2 (EGR2), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: EGR2 early growth response 2 [ Homo sapiens ]
• Official Symbol: EGR2
• Synonyms: EGR2; early growth response 2; early growth response 2 (Krox 20 homolog, Drosophila) , KROX20; E3 SUMO-protein ligase EGR2; Krox 20 homolog; Drosophila; zinc finger protein Krox-20; early growth response protein 2; KROX-20, Drosophila, homolog (early growth response-2); AT591; CMT1D; CMT4E; KROX20; FLJ14547; DKFZp686J1957
• Gene ID: 1959
• mRNA Refseq: NM_000399
• Protein Refseq: NP_000390
• MIM: 129010
• UniProt ID: P11161
• Chromosome Location: 10q21.1
• Pathway: Adipogenesis, organism-specific biosystem; Calcineurin-regulated NFAT-dependent transcription in lymphocytes, organism-specific biosystem; Developmental Biology, organism-specific biosystem; HTLV-I infection, organism-specific biosystem; HTLV-I infection, conserved biosystem; IL4-mediated signaling events, organism-specific biosystem; Transcriptional Regulation of White Adipocyte Differentiation, organism-specific biosystem
• Function: DNA binding; RNA polymerase II activating transcription factor binding; chromatin binding; ligase activity; metal ion binding; protein binding; sequence-specific DNA binding transcription factor activity; transcription regulatory region DNA binding; ubiquitin protein ligase binding; zinc ion binding