Creative BioMart to Present at
                        BIO-Europe Spring Creative BioMart to Present at AACR Annual Meeting|Apr. 5-10, 2024|Booth #2953

Recombinant Human DNAJB1 293 Cell Lysate

Cat.No. : DNAJB1-6892HCL
  • Specification
  • Gene Information
  • Related Products
Description : Antigen standard for DnaJ (Hsp40) homolog, subfamily B, member 1 (DNAJB1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : DNAJB1 DnaJ (Hsp40) homolog, subfamily B, member 1 [ Homo sapiens ]
Official Symbol : DNAJB1
Synonyms : DNAJB1; DnaJ (Hsp40) homolog, subfamily B, member 1; HSPF1; dnaJ homolog subfamily B member 1; Hsp40; radial spoke 16 homolog B (Chlamydomonas); RSPH16B; Sis1; hDj-1; human DnaJ protein 1; heat shock protein 40; dnaJ protein homolog 1; heat shock 40kD protein 1; radial spoke 16 homolog B; heat shock 40 kDa protein 1; DnaJ (Hsp40) homolog, subfmaily B, member 1; Hdj1;
Gene ID : 3337
mRNA Refseq : NM_006145
Protein Refseq : NP_006136
MIM : 604572
UniProt ID : P25685
Chromosome Location : 19p13.2
Pathway : Influenza A, organism-specific biosystem; Influenza A, conserved biosystem; Protein processing in endoplasmic reticulum, organism-specific biosystem; Protein processing in endoplasmic reticulum, conserved biosystem;
Function : heat shock protein binding; unfolded protein binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Inquiry

  • Q&As
  • Reviews

Q&As (7)

Ask a question
How does DNAJB1 contribute to the pathogenesis of neurodegenerative diseases, such as Alzheimer's and Parkinson's? 05/30/2022

DNAJB1 plays a role in neurodegenerative diseases by influencing the handling of misfolded proteins in neurons.

How does DNAJB1 influence the immune response, especially in regulating inflammation and immune cell function? 05/26/2021

DNAJB1 modulates the immune system, particularly in controlling inflammation and immune cell responses.

How does DNAJB1 assist in protein folding and prevent misfolding or aggregation of proteins within cells? 05/16/2021

DNAJB1 aids in protein folding and prevents the aggregation of misfolded proteins, crucial for maintaining cellular proteostasis.

What are the mechanisms by which DNAJB1 modulates apoptosis and cell survival under stress? 02/11/2021

DNAJB1 regulates apoptosis and cell survival, especially under conditions of cellular stress.

What is the impact of DNAJB1 on cancer cell survival, proliferation, and response to chemotherapy? 09/06/2019

DNAJB1 affects cancer cell behavior, influencing tumor growth, survival, and sensitivity to treatments.

What role does DNAJB1 play in cellular responses to heat shock and other stress conditions? 05/08/2019

DNAJB1 is key in cellular stress responses, particularly to heat shock, by stabilizing proteins and aiding in recovery.

How do alterations in DNAJB1 expression or function affect muscle cell health and the development of muscular disorders? 10/04/2018

Changes in DNAJB1 expression impact muscle cell function and health, potentially contributing to muscular disorders.

Customer Reviews (3)

Write a review
Reviews
07/15/2022

    Efficient peptide synthesis, crucial for advancing our peptide research.

    07/21/2021

      Exceptional protein engineering services, a vital resource for our lab.

      11/26/2020

        Reliable protein crystallography, a crucial part of structural research.

        Ask a Question for All DNAJB1 Products

        Required fields are marked with *

        My Review for All DNAJB1 Products

        Required fields are marked with *

        0

        Inquiry Basket

        cartIcon
        logo

        FOLLOW US

        Terms and Conditions        Privacy Policy

        Copyright © 2024 Creative BioMart. All Rights Reserved.

        Contact Us

        • /

        Stay Updated on the Latest Bioscience Trends