Recombinant Human DDIT3 293 Cell Lysate

• Cat.No.: DDIT3-7027HCL

Specification

• Description: Antigen standard for DNA-damage-inducible transcript 3 (DDIT3) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: DDIT3 DNA-damage-inducible transcript 3 [ Homo sapiens ]
• Official Symbol: DDIT3
• Synonyms: DDIT3; DNA-damage-inducible transcript 3; DNA damage-inducible transcript 3 protein; C/EBP zeta; CHOP; CHOP10; GADD153; DDIT-3; c/EBP-homologous protein 10; CCAAT/enhancer-binding protein homologous protein; growth arrest and DNA damage-inducible protein GADD153; CEBPZ; CHOP-10; MGC4154
• Gene ID: 1649
• mRNA Refseq: NM_001195053
• Protein Refseq: NP_001181982
• MIM: 126337
• UniProt ID: P35638
• Chromosome Location: 12q13.1-q13.2
• Pathway: ATF-2 transcription factor network, organism-specific biosystem; Activation of Chaperone Genes by ATF6-alpha, organism-specific biosystem; Activation of Chaperones by ATF6-alpha, organism-specific biosystem; Activation of Genes by ATF4, organism-specific biosystem; Adipogenesis, organism-specific biosystem; Diabetes pathways, organism-specific biosystem; Disease, organism-specific biosystem
• Function: DNA binding; protein dimerization activity; sequence-specific DNA binding; sequence-specific DNA binding transcription factor activity; transcription corepressor activity; transcription factor binding