Recombinant Human Fms-related Tyrosine Kinase 4, 7 Domains, Fc-tagged
Cat.No. : | FLT4-85H |
Product Overview : | Recombinant Human Soluble Human Fms-related Tyrosine Kinase 4 fused with the Fc part of human IgG1 produced in baculovirus is a monomeric, glycosylated, polypeptide containing 774 amino acids and having a molecular mass of 260 kDa. The soluble receptor protein contains only the first 7 extracellular domains, which contain all the information necessary for ligand binding. The VEGFR3 Fc Chimera is purified by proprietary chromatographic techniques. |
- Specification
- Gene Information
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Cat. No. : | FLT4-85H |
Description : | All three VEGF receptors belong to the class III subfamily of receptor tyrosine kinases (RTKs) characterised by the seven immunoglobulin-like loops in the extracellular domain. The expression of VEGFR-1 to -3 is almost exclusively restricted to hematopoietic precursor cells, vascular and lymphatic endothelial cells and to the monocyte/macrophage lineage. They play key roles in vasculogenesis, hematopoiesis, angiogenesis and lymphangiogenesis. The FLT-4 cDNA encodes a 1298 amino acid (aa) residue precursor protein with a 23 aa residue signal peptide. Mature VEGFR-3/FLT-4 is composed of a 751 aa residue extracellular domain, a 22 aa transmembrane domain and a 482 aa residue cytoplasmic domain. Both VEGF family members VEGF-C and VEGF-D have been shown to bind and activate VEGFR-3/FLT-4. The Flt-4 gene is widely expressed in the early embryo but becomes restricted to the lymphatic endothelial a latter stages of development. It is important for lymphangiogenesis. |
Source : | Insect Cells |
Physical Appearance : | Sterile Filtered White lyophilized (freeze-dried) powder. |
Purity : | Greater than 90.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
Formulation : | FLT-4 Fc Chimera was lyophilized from a concentrated (1 mg/ml) sterile solution containing no additives. |
Solubility : | It is recommended to reconstitute the lyophilized FLT4 Fc Chimera in sterile water not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. |
Biological Activity : | Measured by its ability to bind recombinant rat VEGF-C in a functional solid phase binding assay. Immobilised recombinant human VEGFR-3/FLT-4 at 5 µg/ml can bind recombinant rat VEGF-C in a linear range of 8-500 ng/ml. |
Storage : | Lyophilized sVEGFR3 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution VEGFR-3 should be stored at 4°Cbetween 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |
Gene Name : | FLT4 fms-related tyrosine kinase 4 [ Homo sapiens ] |
Synonyms : | FLT4; fms-related tyrosine kinase 4; PCL; FLT41; LMPH1A; VEGFR3; soluble VEGFR3 variant 1; soluble VEGFR3 variant 2; soluble VEGFR3 variant 3; vascular endothelial growth factor receptor 3; EC 2.7.10.1; 3` partial; Tyrosine-protein kinase receptor FLT4; VEGFR-3 |
Gene ID : | 2324 |
mRNA Refseq : | NM_002020 |
Protein Refseq : | NP_002011 |
MIM : | 136352 |
UniProt ID : | P35916 |
Chromosome Location : | 5q34-q35 |
Pathway : | Cytokine-cytokine receptor interaction; Focal adhesion; Signaling by VEGF |
Function : | ATP binding; growth factor binding; nucleotide binding; receptor activity; transferase activity; vascular endothelial growth factor receptor activity |
Products Types
◆ Recombinant Protein | ||
FLT4-101H | Recombinant Human FLT4 Protein (ECD), Fc-His-tagged(C-ter) | +Inquiry |
FLT4-478H | Recombinant Human FLT4 Protein, Fc-tagged | +Inquiry |
FLT4-4377H | Active Recombinant Human FLT4 Protein, GST/His-tagged | +Inquiry |
FLT4-4378H | Active Recombinant Human FLT4 Protein, GST-tagged | +Inquiry |
FLT4-1317H | Recombinant Human FLT4 Protein (M800-Y1363), Tag Free | +Inquiry |
◆ Lysates | ||
FLT4-2016MCL | Recombinant Mouse FLT4 cell lysate | +Inquiry |
FLT4-2709HCL | Recombinant Human FLT4 cell lysate | +Inquiry |
◆ Assay kits | ||
Kit-1594 | Flt4 Activity Assay Kit | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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