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Recombinant Human CYP1A1 293 Cell Lysate

Cat.No. : CYP1A1-7126HCL
  • Specification
  • Gene Information
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Description : Antigen standard for cytochrome P450, family 1, subfamily A, polypeptide 1 (CYP1A1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : CYP1A1 cytochrome P450, family 1, subfamily A, polypeptide 1 [ Homo sapiens ]
Official Symbol : CYP1A1
Synonyms : CYP1A1; cytochrome P450, family 1, subfamily A, polypeptide 1; CYP1, cytochrome P450, subfamily I (aromatic compound inducible), polypeptide 1; cytochrome P450 1A1; CP11; P1 450; P450 C; P450DX; CYPIA1; cytochrome P450-C; cytochrome P450-P1; cytochrome P450 form 6; xenobiotic monooxygenase; aryl hydrocarbon hydroxylase; flavoprotein-linked monooxygenase; cytochrome P1-450, dioxin-inducible; cytochrome P450, subfamily I (aromatic compound-inducible), polypeptide 1; AHH; AHRR; CYP1; P1-450; P450-C;
Gene ID : 1543
mRNA Refseq : NM_000499
Protein Refseq : NP_000490
MIM : 108330
UniProt ID : P04798
Chromosome Location : 15q24.1
Pathway : Biological oxidations, organism-specific biosystem; Cytochrome P450 - arranged by substrate type, organism-specific biosystem; Fatty Acid Omega Oxidation, organism-specific biosystem; Fatty acid, triacylglycerol, and ketone body metabolism, organism-specific biosystem; Metabolic pathways, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of lipids and lipoproteins, organism-specific biosystem;
Function : aromatase activity; demethylase activity; electron carrier activity; enzyme binding; flavonoid 3-monooxygenase activity; heme binding; metal ion binding; oxidoreductase activity; oxidoreductase activity, acting on diphenols and related substances as donors; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen; oxygen binding; steroid hydroxylase activity; vitamin D 24-hydroxylase activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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