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Recombinant Human CYCS 293 Cell Lysate

Cat.No. : CYCS-7135HCL
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Description : Antigen standard for cytochrome c, somatic (CYCS), nuclear gene encoding mitochondrial protein is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : CYCS cytochrome c, somatic [ Homo sapiens ]
Official Symbol : CYCS
Synonyms : CYCS; cytochrome c, somatic; cytochrome c; HCS; CYC; THC4;
Gene ID : 54205
mRNA Refseq : NM_018947
Protein Refseq : NP_061820
MIM : 123970
UniProt ID : P99999
Chromosome Location : 7p21.2
Pathway : Activation of caspases through apoptosome-mediated cleavage, organism-specific biosystem; Alzheimers disease, organism-specific biosystem; Alzheimers disease, conserved biosystem; Amyotrophic lateral sclerosis (ALS), organism-specific biosystem; Amyotrophic lateral sclerosis (ALS), conserved biosystem; Apoptosis, organism-specific biosystem; Apoptosis, organism-specific biosystem;
Function : electron transporter, transferring electrons from CoQH2-cytochrome c reductase complex and cytochrome c oxidase complex activity; heme binding; metal ion binding; protein binding; contributes_to protein serine/threonine phosphatase activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (7)

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How is the release of CYCS from mitochondria regulated? 12/07/2022

Bcl-2 family proteins regulate CYCS release, balancing pro- and anti-apoptotic signals.

What is the role of CYCS in apoptosis? 08/21/2022

In apoptosis, CYCS activates caspases after being released into the cytosol.

How does CYCS contribute to mitochondrial function? 04/16/2022

It transports electrons in the mitochondrial respiratory chain, aiding ATP production.

What happens when there is dysregulation in CYCS activity? 03/19/2022

Dysregulated CYCS activity can lead to impaired cellular energy production or excessive cell death.

What is the primary biological role of CYCS in cellular processes? 09/06/2021

CYCS is crucial for electron transport in mitochondria and inducing apoptosis.

How is CYCS implicated in various diseases? 03/28/2021

Alterations in CYCS are linked to various diseases, including neurodegenerative conditions and cancer.

Are there any therapeutic strategies targeting CYCS? 11/07/2018

Therapeutic strategies may aim to modulate CYCS activity, influencing apoptosis in diseases like cancer.

Customer Reviews (3)

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Reviews
05/07/2022

    Excellent for cell experiments.

    07/01/2021

      Good concentration, easy handling.

      12/03/2020

        Quick shipping, great condition.

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