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Recombinant Human CNR1 293 Cell Lysate

Cat.No. : CNR1-7395HCL
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  • Gene Information
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Description : Antigen standard for cannabinoid receptor 1 (brain) (CNR1), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : CNR1 cannabinoid receptor 1 (brain) [ Homo sapiens ]
Official Symbol : CNR1
Synonyms : CNR1; cannabinoid receptor 1 (brain); CNR; cannabinoid receptor 1; CANN6; CB R; CB1; CB1A; CB1K5; central cannabinoid receptor; CB-R; CB1R;
Gene ID : 1268
mRNA Refseq : NM_001160226
Protein Refseq : NP_001153698
MIM : 114610
UniProt ID : P21554
Chromosome Location : 6q14-q15
Pathway : Class A/1 (Rhodopsin-like receptors), organism-specific biosystem; G alpha (i) signalling events, organism-specific biosystem; GPCR downstream signaling, organism-specific biosystem; GPCR ligand binding, organism-specific biosystem; GPCRs, Class A Rhodopsin-like, organism-specific biosystem; N-cadherin signaling events, organism-specific biosystem; Neuroactive ligand-receptor interaction, organism-specific biosystem;
Function : cannabinoid receptor activity; drug binding; receptor activity; signal transducer activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (6)

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What are the mechanisms of action of CNR1 receptor agonists and antagonists? 07/17/2022

The protein receptor agonists can bind to receptors, activate receptors, and promote their signal transduction, resulting in physiological effects. CNR1 receptor antagonists, on the other hand, can bind to the receptor and prevent endocannabinoids from binding to the receptor, thereby inhibiting its signal transduction.

What are the types of CNR1 mutations? 06/10/2021

There are many types of mutations in the CNR1 gene, including single nucleotide substitution, insertion, or deletion. These mutations may cause structural and functional changes in the CNR1 receptor, affecting its binding and signal transduction to endocannabinoids.

What role does the CNR1 receptor play in neuronal connections and neural circuits? 03/10/2021

CNR1 receptors play an important role in neuronal connections and neural circuits. It is involved in the growth, differentiation, and synapse formation of neurons, as well as in the regulation of neural circuits and signal transduction.

How about the potential applications of the CNR1 receptor in drug development? 08/22/2020

CNR1 receptors have potential applications in drug development. Some studies have shown that CNR1 receptor agonists or antagonists can be used to treat neurological disorders such as pain, depression, and anxiety.

Does the CNR1 gene polymorphism affect individual differences? 08/11/2020

Polymorphisms in the CNR1 gene may cause differences in individual responses to medications. Some polymorphisms may increase an individual's sensitivity or tolerance to medications, which can affect the efficacy and safety of medications.

Do environmental factors influence CNR1 gene expression? 12/01/2019

For example, some drugs, toxins, or stressors may affect the expression level of the CNR1 gene, thus affecting its function and signal transduction processes.

Customer Reviews (3)

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Reviews
08/18/2022

    DNA binding ability is strong, and it performs well in related experiments.

    04/24/2022

      The immunogenicity is very weak, does not cause an immune response, and has a high safety profile.

      04/09/2022

        CNR1 has a long shelf life and good stability, which is convenient for the continuity of subsequent experiments.

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