Recombinant Human CACNB1 293 Cell Lysate

• Cat.No.: CACNB1-7903HCL

Specification

• Description: Antigen standard for calcium channel, voltage-dependent, beta 1 subunit (CACNB1), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: CACNB1 calcium channel, voltage-dependent, beta 1 subunit [ Homo sapiens ]
• Official Symbol: CACNB1
• Synonyms: CACNB1; calcium channel, voltage-dependent, beta 1 subunit; CACNLB1; voltage-dependent L-type calcium channel subunit beta-1; calcium channel, L type, beta 1 polypeptide; calcium channel voltage-dependent subunit beta 1; dihydropyridine-sensitive L-type, calcium channel beta-1 subunit; CAB1; CCHLB1; MGC41896
• Gene ID: 782
• mRNA Refseq: NM_000723
• Protein Refseq: NP_000714
• MIM: 114207
• UniProt ID: Q02641
• Chromosome Location: 17q21-q22
• Pathway: Arrhythmogenic right ventricular cardiomyopathy (ARVC), organism-specific biosystem; Arrhythmogenic right ventricular cardiomyopathy (ARVC), conserved biosystem; Axon guidance, organism-specific biosystem; Calcium Regulation in the Cardiac Cell, organism-specific biosystem; Cardiac muscle contraction, organism-specific biosystem; Cardiac muscle contraction, conserved biosystem; Depolarization of the Presynaptic Terminal Triggers the Opening of Calcium Channels, organism-specific biosystem
• Function: high voltage-gated calcium channel activity; voltage-gated calcium channel activity; voltage-gated ion channel activity