Recombinant Human CA2 293 Cell Lysate
Cat.No. : | CA2-7915HCL |
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Description : | Antigen standard for carbonic anhydrase II (CA2) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection. |
Source : | HEK 293 cells |
Species : | Human |
Components : | This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol). |
Size : | 0.1 mg |
Storage Instruction : | Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment. |
Applications : | ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane. |
Gene Name : | CA2 carbonic anhydrase II [ Homo sapiens ] |
Official Symbol : | CA2 |
Synonyms : | CA2; carbonic anhydrase II; carbonic anhydrase 2; CA II; CAII; Car2; CAC; carbonic anhydrase B; carbonic anhydrase C; carbonic dehydratase; carbonate dehydratase II; CA-II; |
Gene ID : | 760 |
mRNA Refseq : | NM_000067 |
Protein Refseq : | NP_000058 |
MIM : | 611492 |
UniProt ID : | P00918 |
Chromosome Location : | 8q22 |
Pathway : | Bile secretion, organism-specific biosystem; Bile secretion, conserved biosystem; Collecting duct acid secretion, organism-specific biosystem; Collecting duct acid secretion, conserved biosystem; Gastric acid secretion, organism-specific biosystem; Gastric acid secretion, conserved biosystem; Pancreatic secretion, organism-specific biosystem; |
Function : | carbonate dehydratase activity; lyase activity; metal ion binding; zinc ion binding; |
Products Types
◆ Recombinant Protein | ||
CA2-0237H | Active Recombinant Human CA2 Protein | +Inquiry |
CA2-0238H | Recombinant Human CA2 Protein, GST-Tagged | +Inquiry |
CA2-1158M | Recombinant Mouse CA2 Protein, His (Fc)-Avi-tagged | +Inquiry |
CA2-716R | Recombinant Rat CA2 Protein, His (Fc)-Avi-tagged | +Inquiry |
Car2-765M | Recombinant Mouse Car2 Protein, MYC/DDK-tagged | +Inquiry |
◆ Native Protein | ||
CA2-32S | Native Sheep Carbonic Anhydrase II (CA2) Protein | +Inquiry |
CA2-33R | Native Rat Carbonic Anhydrase II (CA2) Protein | +Inquiry |
CA2-30H | Native Human Carbonic Anhydrase II (CA2) Protein | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (7)
Ask a questionCA2 deficiency is a rare autosomal recessive disorder with metabolic acidosis and osteopetrosis.
Acetazolamide is a known inhibitor, used clinically for glaucoma and altitude sickness.
Some mutations can lead to CA2 deficiency, affecting acid-base balance and leading to metabolic acidosis.
CA2 is one of the most efficient and fastest enzymes, displaying higher activity than many other isoforms.
CA2 accelerates the reversible conversion of CO2 and water to bicarbonate and protons, aiding in pH regulation.
CA2 plays a crucial role in CO2 transport and acid-base balance in various tissues.
CA2 is ubiquitously expressed but is particularly abundant in red blood cells and certain epithelial tissues.
Customer Reviews (3)
Write a reviewEfficient sample processing, a time-saving boon for researchers.
Professional expertise, elevates our protein research projects.
Invaluable assistance for intricate protein experiments.
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