Recombinant Human ACAD8, T7-tagged

• Cat.No.: ACAD8-26H
• Product Overview: Recombinant Human Acyl-Coenzyme A Dehydrogenase 8 produced inE.Coliis a non-glycosylated, polypeptide chain containing amino acids 1-415 and having a total molecular mass of 47.7 kda. ACAD8 contains T7 tag at the N-terminus. ACAD-8 is purified by proprietary chromatographic techniques.

Specification

• Cat. No.: ACAD8-26H
• Description: Acyl CoA dehydrogenase is the enzyme used to catalyze the first step of β-oxidation in Fatty acid metabolism. Acyl-coenzyme A (CoA) dehydrogenases (ACADs) are a family of mitochondrial enzymes that catalyze the first dehydrogenation step in the bets-oxidation of fatty acyl-CoA derivatives. Several human ACADs exist and all ACADs catalyze the same initial dehydrogenation of the substrate at the beta-carbon atom and require electron transfer flavoprotein as an alectron acceptor. The predicted 415-amino acid ACAD8 protein contains many of the residues conserved in most other ACADs, including an active site glutamic acid residue and residues important for tetramer formation.
• Source: Escherichia Coli.
• Physical Appearance: Sterile Filtered clear solution.
• Purity: Greater than 95.0% as determined by SDS-PAGE.
• Formulation: Acyl-Coenzyme A Dehydrogenase 8 at a concentration of 0.1 mg/ml in 10 mM Tris, pH 8.0, 0.1% Triton X-100, 0.002% NaN3, 10 mM DTT.
• Applications: • ELISA • MS • Inhibition Assays • Western Blotting
• Stability: ACAD8 although stable at 14°C for 1 week, should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Gene Information

• Gene Name: ACAD8 acyl-Coenzyme A dehydrogenase family, member 8 [ Homo sapiens ]
• Synonyms: ACAD8; acyl-Coenzyme A dehydrogenase family, member 8; ACAD-8; FLJ22590; Isobutyryl-CoA dehydrogenase, mitochondrial;Acyl-CoA dehydrogenase family member 8; Activator-recruited cofactor 42 kDa component; ARC42
• Gene ID: 27034
• mRNA Refseq: NM_014384
• Protein Refseq: NP_055199
• UniProt ID: Q9UKU7
• Chromosome Location: 11q25
• MIM: 604773
• Pathway: Metabolic pathways; Valine, leucine and isoleucine degradation; Metabolism of amino acids
• Function: FAD binding; acyl-CoA dehydrogenase activity