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Recombinant Human ART1 293 Cell Lysate

Cat.No. : ART1-8672HCL
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  • Gene Information
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Description : Antigen standard for ADP-ribosyltransferase 1 (ART1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : ART1 ADP-ribosyltransferase 1 [ Homo sapiens ]
Official Symbol : ART1
Synonyms : ART1; ADP-ribosyltransferase 1; GPI-linked NAD(P)(+)--arginine ADP-ribosyltransferase 1; ART2; CD296; ADP-ribosyltransferase 2; mono(ADP-ribosyl)transferase 1; RT6; MGC133217;
Gene ID : 417
mRNA Refseq : NM_004314
Protein Refseq : NP_004305
MIM : 601625
UniProt ID : P52961
Chromosome Location : 11p15
Pathway : Alpha-defensins, organism-specific biosystem; Defensins, organism-specific biosystem; Immune System, organism-specific biosystem; Innate Immune System, organism-specific biosystem;
Function : NAD(P)+-protein-arginine ADP-ribosyltransferase activity; NAD+ ADP-ribosyltransferase activity; transferase activity, transferring glycosyl groups;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (7)

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How does ART1 carry out ADP-ribosylation? 10/10/2022

ART1 transfers ADP-ribose from nicotinamide adenine dinucleotide (NAD+) to specific arginine residues on substrate proteins, modifying their structure and function.

Are there any known substrates that ART1 acts upon? 01/22/2020

ART1 predominantly ADP-ribosylates arginine residues on G protein-coupled receptors (GPCRs), such as the P2Y purinergic receptor.

Are there any known natural inhibitors or regulators of ART1 activity? 07/31/2017

Some pathogens produce enzymes called ADP-ribosyltransferase toxins that can inhibit ART1 activity and evade the immune response.

In which tissues or organs is ART1 predominantly expressed? 07/29/2017

ART1 is primarily expressed in the testes, but it can also be found in other tissues such as the spleen, kidney, and liver.

Does ART1 have any non-enzymatic functions or interactions with other proteins? 01/07/2017

Aside from its enzymatic role in ADP-ribosylation, ART1 does not have any known non-enzymatic functions or specific protein interactions.

Are there any known diseases or disorders associated with mutations in the ART1 gene? 11/22/2016

Mutations in the ART1 gene have not been directly linked to specific diseases or disorders in humans so far.

Is the activity of ART1 influenced by any cofactors or regulators? 05/24/2016

The activity of ART1 is dependent on the presence of NAD+ as a substrate, and it can be modulated by other factors, including pH and the presence of certain ions.

Customer Reviews (3)

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Reviews
01/16/2022

    One aspect that sets the ART1 protein apart is the excellent technical support provided by the manufacturer.

    01/18/2020

      Its purity and reliability have consistently resulted in successful outcomes in my research projects, providing me with accurate and reproducible data.

      03/23/2016

        The ART1 protein has demonstrated remarkable stability, allowing for extended storage without compromising its quality.

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